The protein samples were subjected to 7.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by immunoblot analysis. The blots were incubated overnight at 4 °C with a rabbit polyclonal anti-cofilin antibody (1:1000; Millipore), polyclonal anti-p-cofilin antibody (1:1000; Millipore), mouse monoclonal anti-PSD95 (1:1000; Cell Signaling Technology), monoclonal anti-GluR1 antibody (1:1000; Cell Signaling Technology, Inc, Danvers, MA, USA) or monoclonal anti–GAPDH antibody (1:500; Santa Cruz Biotechnology Inc). The membranes were washed extensively and then incubated with horseradish peroxidase-conjugated anti-mouse and anti-rabbit IgG antibody (1:10,000; Jackson ImmunoResearch Laboratories Inc). The immunoreactivity was detected using enhanced chemiluminescence (ECL Advance Kit; Amersham Biosciences). The intensity of the bands was captured digitally and analyzed quantitatively using ImageJ software. The immunoreactivity of target proteins was normalized to that of GAPDH.
Mouse monoclonal anti psd95
Manufactured by Cell Signaling Technology
Sourced in United States
Mouse monoclonal anti-PSD95 is a laboratory reagent used to detect and localize the PSD95 protein in biological samples. PSD95 is a scaffolding protein found in the postsynaptic density of neurons and is involved in the organization of the postsynaptic signaling complex.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh monoclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Horseradish peroxidase conjugated anti mouse and anti rabbit igg antibody, by Jackson ImmunoResearch (1 mentions)
Ecl advance kit, by Cytiva (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal anti β tubulin, by Merck Group (1 mentions)
Tritc labeled phalloidin, by Merck Group (1 mentions)
2 protocols using mouse monoclonal anti psd95
1
Immunoblot Analysis of Synaptic Proteins
2
Comprehensive Neuronal Marker Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mouse monoclonal anti-β-tubulin (used 1:150) was obtained from Sigma-Aldrich. Monoclonal anti-NeuN/Fox3 (used 1:150) produced in mouse primary antibody was purchased from Immunological Sciences. Rabbit monoclonal anti-Synapsin 1 (used 1:200), rabbit monoclonal anti-Synaptophysin (used 1:100), and rabbit monoclonal anti-Complexin 1/2 (used 1:800) were purchased from Cell Signaling (Synaptic Neuron Marker Antibody Sampler kit). Mouse monoclonal anti-PSD95 (used 1:100) was obtained from Cell Signaling. TRITC-labeled phalloidin (used 1:1000) was from Sigma Aldrich. Alexa Fluor 488 mouse anti-Choline Acetyltransferase (ChaT) (used 1:100) was obtained from Abcam. Mouse monoclonal anti-Tyrosine Hydroxylase (TH) (used 1:300) was purchased from Cell Signaling. Alexa Fluor 488 goat anti-rabbit (used 1:200), and Cy3 goat anti-mouse (used 1:200) were purchased from Life Technologies. PhenoVue 641 Mitochondrial Stain (used 100 nM) was obtained from PerkinElmer. Hoechst 33342 (working concentration 1 µg/mL) was purchased from ThermoFisher.
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