Cell migration and invasion assays were performed using transwell plates with 8‐μm‐pore filters (Corning, NY), following the manufacturer's protocol. Briefly, for the cell migration assay, transfected Hey, SKOV3, and HO8910 cells (2 × 105) were suspended in 200 μL of serum‐free medium and seeded into the upper chambers of the transwell plates, and medium supplemented with 20% fetal bovine serum was applied to the lower chamber as a chemoattractant to induce migration. After incubation for 12–24 h at 37°C, tumor cells were fixed with 4% cold paraformaldehyde and stained with crystal violet (Beyotime, Shanghai, China). For the invasion assay, the procedures were conducted as described above, except that filter inserts were coated with BD Matrigel and the plates were cultured for 16–24 h at 37°C. Migrated cells were counted at 100× magnification under an inverted microscope. Independent experiments were performed in triplicate.
Transwell plates with 8 μm pore filters
Manufactured by Corning
Sourced in United States
Transwell plates with 8-μm-pore filters are a type of cell culture insert used for various in vitro cell migration, invasion, and co-culture studies. The plates feature a membrane with 8-micron pores that allow the passage of cells and materials between the upper and lower chambers.
Automatically generated - may contain errors
3 protocols using transwell plates with 8 μm pore filters
1
Transwell Assay for Cell Migration and Invasion
2
In Vitro Angiogenesis and Proliferation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tube formation assay was conducted to evaluate angiogenesis in vitro using a Matrigel basement membrane matrix (356,234; BD Biosciences, CA, USA) following the manufacturer's protocols, and the Transwell assay was conducted to evaluate cell proliferation. Briefly, Matrigel (50 μL/well) was added to 96-well plates using cold pipette tips on ice after overnight thawing at 4 °C. Then, the plates were incubated at 37 °C until the Matrigel became solidified. Next, HUVECs (5 × 104 cells/well) were co-cultured with the extracts from the PCL scaffold, PCL-GelMA scaffold, or the blank groups. Tube formation was evaluated using an inverted microscope after incubation at 37 °C for 6 h. The total length of the tubes was measured using ImageJ software (Media Cybernetics, MD, USA).
HUVECs (1 × 105 cells/well) were co-cultured with the extracts of PCL scaffold or PCL-GelMA scaffold and seeded in the upper chambers of 24-well Transwell plates with 8-μm pore filters (Corning, NY, USA) to perform the Transwell assay. After incubation at 37 °C for 24 h, the cells on the lower surface were stained with 0.1% crystal violet for several minutes after removing the attached cells on the upper surface of the filter membranes. The extent of cell migration was observed using the optical microscope (Leica, Solms, Germany).
HUVECs (1 × 105 cells/well) were co-cultured with the extracts of PCL scaffold or PCL-GelMA scaffold and seeded in the upper chambers of 24-well Transwell plates with 8-μm pore filters (Corning, NY, USA) to perform the Transwell assay. After incubation at 37 °C for 24 h, the cells on the lower surface were stained with 0.1% crystal violet for several minutes after removing the attached cells on the upper surface of the filter membranes. The extent of cell migration was observed using the optical microscope (Leica, Solms, Germany).
3
Transwell Assay for Cell Migration and Invasion
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell migration and invasion were assayed using Transwell plates with 8‐μm pore filters (Corning, NY). The procedure was performed according to the manufacturer's protocol. Briefly, for the cell migration assay, transfected HEY, SKOV3, and HO8910 (2 × 105) cells were suspended in 200 μl of serum‐free DMEM and plated into the upper chambers. Then, 600 μl of DMEM/F‐12 supplemented with 10% FBS was added to the lower chamber. After incubation for 12–14 h at 37°C, the tumor cells were fixed with 4% cold paraformaldehyde and stained with crystal violet (Beyotime, Shanghai, China). For the invasion assay, the procedures were conducted as described earlier, except the filter inserts were coated with BD Matrigel and the plates were incubated for 16–20 h at 37°C. Cells that had migrated were counted at 100× magnification under an inverted microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!