Isolates were inoculated onto sheep blood agar and incubated for 24 h at 37 °C. Bacterial colonies were applied onto a 96-spot target plate and allowed to dry at room temperature. Subsequently, 2 μl of MALDI matrix [a saturated solution of α-cyano-4-hydroxycinnamic acid (HCCA) in 50% acetonitrile and 2.5% trifluoroacetic acid] was applied onto the colonies and allowed to dry before testing. Then the target plate was loaded into the MALDI-TOF MS instrument (MicroFlex LT mass spectrometer, Bruker Daltonics). Spectra were analyzed using MALDI Biotyper automation control and the Bruker Biotyper 2.0 software and library (version 2.0, 3,740 entries; Bruker Daltonics). Identification score criteria used were those recommended by the manufacturer: a score of ≥ 2.000 indicated species-level identification, a score of 1.700 to 1.999 indicated identification to the genus level, and a score of <1.700 was interpreted as no identification. Isolates that failed to produce a score of <1.700 with direct colony or extraction methods were retested. S. aureus ATCC25923, E. coli ATCC25922 and Pseudomonas aeruginosa ATCC27853 were used as controls.
Maldi biotyper automation control
Manufactured by Bruker
Sourced in Germany
The MALDI Biotyper automation control is a software component that enables automated operation of the MALDI Biotyper system. It provides the necessary functionality to control sample preparation, measurement, and data analysis workflows within the MALDI Biotyper platform.
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Lab products found in correlation
3 protocols using maldi biotyper automation control
1
MALDI-TOF MS Bacterial Identification
2
MALDI-TOF MS Bacterial Identification
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Isolates were inoculated onto sheep blood agar and incubated for 24 h at 37 °C. Bacterial colonies were applied onto a 96-spot target plate and allowed to dry at room temperature. Subsequently, 2 μl of MALDI matrix [a saturated solution of α-cyano-4-hydroxycinnamic acid (HCCA) in 50% acetonitrile and 2.5% trifluoroacetic acid] was applied onto the colonies and allowed to dry before testing. Then the target plate was loaded into the MALDI-TOF MS instrument (MicroFlex LT mass spectrometer, Bruker Daltonics). Spectra were analyzed using MALDI Biotyper automation control and the Bruker Biotyper 2.0 software and library (version 2.0, 3,740 entries; Bruker Daltonics). Identification score criteria used were those recommended by the manufacturer: a score of ≥ 2.000 indicated species-level identification, a score of 1.700 to 1.999 indicated identification to the genus level, and a score of <1.700 was interpreted as no identification. Isolates that failed to produce a score of <1.700 with direct colony or extraction methods were retested. S. aureus ATCC25923, E. coli ATCC25922 and Pseudomonas aeruginosa ATCC27853 were used as controls.
3
Identification of Bacterial Strain L156.4 Using MALDI-TOF
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The identification of the new strain L156.4 was performed using Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF) Mass Spectrometry. The strain was cultured overnight on MRS agar at 37°C in anaerobic conditions. For the analysis, individual samples colonies were scraped up using a sterile plastic loop and then applied as a thin film onto a 24-spot steel plate (Bruker Daltonics, Bremen, Germany). After being air-dried, the sample was co-crystallized with 1 μl of a saturated solution of α-cyano-4-hydroxycinnamic acid matrix (HCCA; Bruker Daltonics, Bremen, Germany) in 50% acetonitrile/2.5% trifluoroacetic acid (Sigma-Aldrich, St. Louis, MO, USA). Mass spectra were acquired in reflector-positive mode on a MicroFlex LT system tabletop instrument (Bruker Daltonics) using the manufacturer's default settings. Captured spectra were analyzed using the MALDI Biotyper automation control and Bruker Biotyper 2.0 software (Bruker Daltonics, Bremen, Germany). The identification criteria used in our analysis were as follows: a score ≥2.000 indicated a species level identification, a score of 1.700 to 1.999 indicated identification at the genus level, and a score <1.700 was interpreted as not identified. Escherichia coli ATCC 8739 was used as a positive control.
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