Irisin elisa

Plasma irisin levels were measured through the competitive enzyme-linked immunosorbent assay (ELISA) method using commercially available kit (Irisin ELISA, Biovendor, Cat. No: RAG018R, Brno, Czech Republic ), according to the manufacturer’s directions. The coefficients of intra- and inter-assay variations were 4.2% (n = 20) and 4.9% (n = 20), respectively.

Blood glucose levels were measured by glucose oxidase assay (GLU GOD, Erba-Lachema, Czech Republic) using tail vein blood drawn into 5% trichloracetic acid and promptly centrifuged. Serum TAG concentrations were quantified using standard enzymatic methods (TG L 250 S, Erba-Lachema, Czech Republic). Non-esterified fatty acid levels (NEFA) were determined using an acyl-CoA oxidase-based colorimetric kit (Roche Diagnostics GmbH, Germany). Serum insulin concentrations were evaluated using a rat insulin enzyme-linked immunosorbent assay kit (Mercodia, Sweden). Irisin serum concentrations were measured using an irisin enzyme-linked immunosorbent assay kit (Irisin ELISA, BioVendor, Czech Republic). The insulin sensitivity index (ISI) was calculated from fasting insulin (FI) and fasting glucose (FG) as described previously [37 (link)]. ISI = 1/(FG*FI), where FG is expressed as mg/dl and FI as mIU/l.