After vaginal bleeding first appeared, blood was collected daily from the cephalic vein, and sera were separated by centrifuging for 10 min. Serum progesterone concentration was monitored by an IMMULITE 1000 (Siemens Healthcare Diagnostics Inc., Flanders, NJ, USA). The day when the progesterone concentration reached 4.0 ng/mL to 10.0 ng/mL was considered the day of ovulation. Seventy-two hours after ovulation, in vivo matured oocytes were collected by oviductal flushing using HEPES-buffered tissue culture medium-199 (TCM, Invitrogen, Carlsbad, CA, USA) supplemented with 10% bovine serum albumin and 2 mM NaHCO3.
Hepes buffered tissue culture medium 199 tcm
Manufactured by Thermo Fisher Scientific
Sourced in United States
HEPES-buffered tissue culture medium-199 (TCM) is a laboratory reagent used in cell culture applications. It provides a balanced salt solution and essential nutrients to support the growth and maintenance of cell lines in vitro. The medium is buffered with HEPES to help maintain a stable pH during cell culture procedures.
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2 protocols using hepes buffered tissue culture medium 199 tcm
1
Ovulation Detection and Oocyte Collection
2
Oocyte Collection via Oviductal Flushing
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After vaginal bleeding was rst shown, blood was collected daily from the cephalic vein and sera were separated by centrifuging for 10 min. Serum progesterone concentration was monitored by an IMMULITE 1000 (Siemens Healthcare Diagnostics Inc., Flanders, NJ, USA). The day when the progesterone concentration reached 4.0 ng/ml to 10.0 ng/ml was considered the day of ovulation. 72 h after ovulation, in vivo matured oocytes were collected by oviductal ushing using HEPES-buffered tissue culture medium-199 (TCM, Invitrogen) supplemented with 10% bovine serum albumin and 2 mM NaHCO3.
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