Atr pro 470 h

FT-IR spectroscopy was used to acquire the characteristic spectra of the cutin samples isolated in both conventional and HPH-assisted extraction processes using an FT-IR-4100 series spectrophotometer (Jasco Europe S.r.l., Cremella, Italy) equipped with a single-reflectance horizontal attenuated total reflectance (ATR) cell (ATR-PRO 470-H, Jasco Europe S.r.l., Cremella, Italy). The infrared spectra were recorded in the 4000–650 cm⁻¹ range at a resolution of 4 cm⁻¹ after 16 scans. The resulting spectrum was based on at least three repetitions, which were averaged and smoothed utilizing the adaptive-smoothing function.

UV–vis absorption spectroscopy (V-570 UV–vis spectrophotometer, Jasco, Hachioji, Japan) was used to determine the optical properties, to confirm the formation of AgNPs into the polyol, and to study the stability of both polyol/AgNPs and PUF/AgNPs. The analyses were performed in the wavelength range of 350–800 nm, using quartz cells with a 1 cm optical path and at a resolution of 1 nm (for liquid samples) and integrating spheres (for foams).
To study the gamma radiation influence on polyol’s and foams’ structure, infrared spectroscopy (FTIR-4200 spectrometer, Jasco, Hachioji, Japan) was used. The spectra were recorded using an ATR PRO470-H (Attenuated Toal Reflectance, JASCO) accessory in the spectral range of 4000–400 cm⁻¹, at a resolution of 4 cm⁻¹, and 200 scans for each spectrum.

Pure MEL, pure polymers, Tween^® 80, physical mixture (PhM) between MEL and pure polymer (MEL + PVA and MEL + PLA) and all nanofiber samples were analyzed using a Jasco FT/IR-4200 spectrophotometer. The instrument was equipped with single reflection accessory (Jasco ATR PRO470-H) detector. The measurements were detected in absorbance mode and background was acquired before of each measure. For each spectrum, 100 scans were collected over the range of 4000 and 500 cm⁻¹, at a resolution of 4 cm⁻¹ at room temperature. Spectra Manager-II software (Jasco, Easton, MD, USA) was used for data acquisition.