Luna omega 5 μm ps c18 100 lc column

All peptides were purified using preparative reversed-phase high-performance liquid chromatography (HPLC) on an Agilent 1260 Infinity II system using a Phenomenex Luna Omega 5 μm PS C18 100 Å LC column. Varying gradients of ACN and 0.1% TFA in H₂O were used to separate the respective peptides. The purity of each peptide was checked using an analytical Agilent 1260 Infinity II system using a Phenomenex Luna Omega 5 μm PS C18 100 Å LC column. Mass spectrometry was performed using a Bruker matrix-assisted laser desorption ionization− time-of-flight (MALDI-TOF) Ultraflex III mass spectrometer and α-Cyano-4-hydroxycinnamic acid (CHCA; Sigma) as the matrix. Peptides were lyophilized using a Labconco FreeZone Plus 12 Liter Cascade Console Freeze-Dry system.

All peptides were purified using preparative reversed-phase high-performance liquid chromatography (HPLC) on an Agilent 1260 Infinity II system using a Phenomenex Luna Omega 5 μm PS C18 100 Å LC column. Varying gradients of ACN and 0.1% TFA in H₂O were used to separate the respective peptides. Purity of each peptide was checked using an analytical Agilent 1260 Infinity II system using a Phenomenex Luna Omega 5 μm PS C18 100 Å LC column. Mass spectrometry was performed using a Bruker matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) Ultraflex III mass spectrometer and α-cyano-4-hydroxycinnamic acid (CHCA; Sigma) as the matrix. Peptides were lyophilized using a Labconco FreeZone Plus 12 Liter Cascade Console Freeze Dry system.