Mouse monoclonal anti mt 1 2 antibody e9

Bovine aortic endothelial cells were purchased from Cell Applications (San Diego, CA, USA). The following materials were purchased from the respective vendors: Dulbecco's modified Eagle's medium (DMEM) and calcium-and magnesium-free phosphate-buffered saline (CMF-PBS; Nissui Pharmaceutical, Tokyo, Japan); fetal bovine serum and a High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific, Waltham, MA, USA); QIAzol lysis reagent (QIAGEN, Valencia, CA, USA); GeneAce SYBR qPCR Mixα (Nippon Gene, Tokyo, Japan); mouse monoclonal anti-MT-1/2 antibody (E9; Dako, Glostrup, Denmark); Mouse monoclonal anti-β-actin antibody (Wako Pure Chemical Industries, Osaka, Japan); horseradish peroxidase-conjugated antimouse IgG antibody (#7076; Cell Signaling, Beverly, MA, USA); May-Grünwald and Giemsa stain solution (Merck KGaA, Darmstadt, Germany); and cadmium chloride, manganese chloride tetrahydrate, and other reagents (Nacalai Tesque, Kyoto, Japan).

Cu10 was purchased from Tokyo Chemical Industry (Tokyo, Japan). Bovine aortic endothelial cells were purchased from Cell Applications (San Diego, CA, USA). Dulbecco's modified Eagle's medium and calcium-and magnesium-free phosphate buffered saline were from Nissui Pharmaceutical (Tokyo, Japan). Fetal bovine serum was from HyClone Laboratories (Waltham, MA, USA). Bicinchoninic acid protein assay reagent kit was from Thermo Fisher Scientific (Waltham, MA, USA). Polyvinylidene difluoride membrane (0.2 μm), sodium diethyldithiocarbamate trihydrate (Na01), and mouse monoclonal anti β-actin antibody were from Wako Pure Chemical Industries (Osaka, Japan). Mouse monoclonal anti-MT-1/2 antibody (E9) was from Dako (Glostrup, Denmark). Rabbit polyclonal anti-human Nrf2 antibody (H-300) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Horseradish peroxidase-conjugated anti-rabbit IgG antibody (#7074) and horseradish peroxidase conjugated anti-mouse IgG antibody (#7076) were from Cell Signaling (Beverly, MA, USA). High-Capacity cDNA Reverse Transcription kit was from Applied Biosystems (Foster, CA, USA). Gene Ace SYBR qPCR Mixα was from Nippon Gene (Tokyo, Japan). Dual-Luciferase Reporter Assay System and pRL-SV40 were from Promega (Madison, WI, USA). Chemi-Lumi One L and other reagents were from Nacalai Tesque (Kyoto, Japan).