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Macs selection column

Manufactured by Miltenyi Biotec
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The MACS Selection Column is a laboratory equipment designed for the magnetic separation and enrichment of cells. It serves as a critical component in the MACS (Magnetic-Activated Cell Sorting) system, enabling the isolation and purification of target cell populations from complex samples. The column is filled with a matrix that facilitates the retention of magnetically labeled cells, allowing for the separation and collection of the desired cell fraction.

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Lab products found in correlation

Facsaria 3 sorter, by BD (2 mentions) Cd45ra apc hi100, by BD (1 mentions) Cd19 bv605 sj25c1, by BD (1 mentions) Cd34 pe antibody, by BD (1 mentions)

Close spelling variants of this product

CD8-negative selection MACS column MACS columns Selection column

2 protocols using macs selection column

1

Isolation of Human CD34+ Cells

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Transplanted human cord blood cells were harvested from mice 14 weeks post transplant. Cells harvested from 5 mice with equivalent engraftment for each condition were pooled and CD34+ cells isolated using the MACS selection column (Miltenyi, cat. #130–046-702). Cells were then stained with antibodies against human CD34-PE (581; BD), CD45RA-APC (HI100; BD), CD38-PC7 (HB7; BD), CD19-BV605 (SJ25C1; BD), CD10-BV786 (HI10a; BD), CD90-Biotin (5E10; BD) and analyzed on a BD FACS Aria III sorter.
Reilly A., Creamer J.P., Stewart S., Stolla M.C., Wang Y., Du J., Wellington R., Busch S., Estey E.H., Becker P.S., Fang M., Keel S.B., Abkowitz J.L., Soma L.A., Ma J., Duan Z, & Doulatov S. (2022). Lamin B1 deletion in myeloid neoplasms causes nuclear anomaly and altered hematopoietic stem cell function. Cell stem cell, 29(4), 577-592.e8.
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2

Purification of Transplanted Human CB Cells

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Transplanted human CB cells were harvested from mice 14 weeks post-transplant. Cells harvested from 5 mice with equivalent engraftment for each condition were pooled and CD34+ cells isolated using the MACS selection column (Miltenyi, cat. #130–046-702). Cells were then stained with the CD34-PE antibody (BD, cat. #555822) and CD34+ cells were further purified by flow sorting on a BD FACS Aria III sorter. Single cell sequencing was performed using the 10X Chromium Next GEM Single Cell 3’ Reagents kit v.3.1 on a Chromium Controller.
Reilly A., Creamer J.P., Stewart S., Stolla M.C., Wang Y., Du J., Wellington R., Busch S., Estey E.H., Becker P.S., Fang M., Keel S.B., Abkowitz J.L., Soma L.A., Ma J., Duan Z, & Doulatov S. (2022). Lamin B1 deletion in myeloid neoplasms causes nuclear anomaly and altered hematopoietic stem cell function. Cell stem cell, 29(4), 577-592.e8.
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