Using a UV visible double beam spectrophotometer, the concentration of BO encapsulated into BO-ICs was determined spectrophotometrically at 254 nm (Cary 3500 UV-Vis Engine, Agilent Technologies, Pty Ltd, Mulgrave, Australia). Briefly, BO-ICs (5 mg) were suspended in acetonitrile (5 mL) and subsequently left in the dark at 24 °C, inside sealed containers while being mixed constantly for 72 h to facilitate the transfer of the BO entrapped to the solution. A calibration curve was developed for the BO, under the same conditions, in a range of concentrations of 3.125 to 200 μg mL−1 (BO: y = 0.0025x – 0.0053 and r2 = 0.9994). The % EE and % DL were then determined using the following eqn (1) and (2) :44 (link)
Cary 3500 uv vis engine
Manufactured by Agilent Technologies
Sourced in Australia
The Cary 3500 UV-Vis Engine is a high-performance spectrophotometer designed for accurate and reliable measurement of ultraviolet and visible light absorption. It provides precise spectral data with a wide wavelength range and advanced optics.
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Lab products found in correlation
2 protocols using cary 3500 uv vis engine
1
Spectrophotometric Quantification of BO Encapsulation
2
Spectrophotometric Quantification of PO Encapsulation
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The amount of PO encapsulated into
their ICs was calculated spectrophotometrically at 256 nm using a
UV–vis double beam spectrophotometer (Cary 3500 UV–vis
Engine, Agilent Technologies Australia (M) Pty Ltd., Mulgrave, Australia).
First, 5 mg of PO-ICs was added to 5 mL of acetonitrile (99% HPLC-
and UV-grade) in a sealed container protected from light. This solution
was then left on an agitating shaker for 72 h at room temperature
to allow enough time for the entrapped PO to be released from the
PO-ICs and transferred to the solution (acetonitrile). After 72 h,
the obtained solution contained the released PO in addition to the
powder of the ICs (the leftover HPβCD) which precipitated at
the bottom. Hence, few milliliters of the obtained solution, which
contained the released PO, were withdrawn to be read spectrophotometrically.
Under the same conditions, a standard curve for PO was prepared with
a range of concentrations between 3.125 and 200 μg/mL (PO: y = 0.0035x + 0.0161, r2 = 0.9997). The following equations (A) and (B) were
used to calculate the %EE and %DL of PO-ICs, given that the EE was
expressed as a w/w percentage of the PO amount entrapped into the
ICs to the initial amount of the PO used for ICs formation:54 (link)
their ICs was calculated spectrophotometrically at 256 nm using a
UV–vis double beam spectrophotometer (Cary 3500 UV–vis
Engine, Agilent Technologies Australia (M) Pty Ltd., Mulgrave, Australia).
First, 5 mg of PO-ICs was added to 5 mL of acetonitrile (99% HPLC-
and UV-grade) in a sealed container protected from light. This solution
was then left on an agitating shaker for 72 h at room temperature
to allow enough time for the entrapped PO to be released from the
PO-ICs and transferred to the solution (acetonitrile). After 72 h,
the obtained solution contained the released PO in addition to the
powder of the ICs (the leftover HPβCD) which precipitated at
the bottom. Hence, few milliliters of the obtained solution, which
contained the released PO, were withdrawn to be read spectrophotometrically.
Under the same conditions, a standard curve for PO was prepared with
a range of concentrations between 3.125 and 200 μg/mL (PO: y = 0.0035x + 0.0161, r2 = 0.9997). The following equations (A) and (B) were
used to calculate the %EE and %DL of PO-ICs, given that the EE was
expressed as a w/w percentage of the PO amount entrapped into the
ICs to the initial amount of the PO used for ICs formation:54 (link)
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