Macrophage proliferation was analyzed using Cell Counting Kit-8 (Dojindo, Japan). Cultured cells (RAW264.7) were dispensed into a 96-well plate at a density of 1000 cells/well, with or without a different level of IL-34 protein (Sino Biological, China) or conditioned medium (CM) from TNBC cells. To assess the IL-34-induced p38 and mTOR signaling pathways, the cells were treated with the CSF-1R inhibitor pexidartinib (AbMole, USA), the p38 inhibitor SB203580 (MedChemExpress, USA), or the mTOR inhibitor AZD8055 (MedChemExpress). At the indicated time points, the absorbance at 450 nm was measured to determine the number of viable cells in each well.
Il 34 protein
Manufactured by Sino Biological
Sourced in China
IL-34 protein is a cytokine that plays a role in the regulation of macrophage differentiation and survival. It binds to the CSF-1 receptor (CSF-1R) and activates downstream signaling pathways. The IL-34 protein is available as a recombinant protein product for research use.
Automatically generated - may contain errors
2 protocols using il 34 protein
1
Macrophage Proliferation Modulation by IL-34
2
Macrophage Proliferation Modulation by IL-34
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Macrophage proliferation was analyzed using Cell Counting Kit-8 (Dojindo, Japan). Cultured cells (RAW264.7) were dispensed into a 96-well plate at a density of 1000 cells/well, with or without a different level of IL-34 protein (Sino Biological, China) or conditioned medium (CM) from TNBC cells. To assess the IL-34-induced p38 and mTOR signaling pathways, the cells were treated with the CSF-1R inhibitor pexidartinib (AbMole, USA), the p38 inhibitor SB203580 (MedChemExpress, USA), or the mTOR inhibitor AZD8055 (MedChemExpress). At the indicated time points, the absorbance at 450 nm was measured to determine the number of viable cells in each well.
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