Anti cd133 293c3 pe

ADSCs were harvested by treatment with 0.25% trypsin/EDTA (Gibco) and then resuspended at a total number of 2 × 10 6 cells in 500 μL PBS. ADSCs were labeled for 15 min at 4°C with the following monoclonal antibodies: anti-CD29-FITC (eBioscience, San Diego, CA, USA), anti-CD34-FITC (BD Biosciences, San Diego, CA, USA), anti-CD44-PE (BD Biosciences), anti-CD90-FITC (BD Biosciences), anti-CD133 (293C3)-PE (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), or HLA-DR-FITC (BD Biosciences). The cells were thoroughly washed with PBS before analysis. FITC-or PE-labeled mouse IgG (Biolegend, San Diego, CA, USA) was used as the isotype control. The cells were then analyzed with a BDFACSCanto flow cytometer (Becton-Dickinson, San Jose, CA, USA). Data were analyzed with the Cell Quest software package (Becton Dickinson, Bedford, Mass, USA).