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Pfrs2 y196

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PFRS2 Y196 is a laboratory reagent produced by Cell Signaling Technology. It is a phospho-specific antibody that recognizes the tyrosine 196 phosphorylation site on the PFRS2 protein.

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Lab products found in correlation

Heparin sulfate, by STEMCELL (1 mentions) Vimentin, by Standard BioTools (1 mentions) Fibroblast growth factor 2 (fgf2), by Cell Signaling Technology (1 mentions) Phospho frs2, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Tgf β, by Cell Signaling Technology (1 mentions) Anti fgfr1, by Cell Signaling Technology (1 mentions) Phospho fgfr, by Cell Signaling Technology (1 mentions) Anti frs2, by Abcam (1 mentions) Phospho plcγ, by Cell Signaling Technology (1 mentions) Pfgfr1, by Cell Signaling Technology (1 mentions) Horseradish peroxidase hrp linked rabbit igg secondary antibody, by Cell Signaling Technology (1 mentions) Hsp90, by Cell Signaling Technology (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) Phospho stat3, by Cell Signaling Technology (1 mentions) Hif 1α, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions)

2 protocols using pfrs2 y196

1

FGF2 and FGFR1 Signaling Pathway Assay

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Recombinant FGF2 was purchased from Gibco (#PHG0024). Heparin sulfate was purchased from StemCell Technologies (#07980). The following antibodies were used (all primary antibodies are rabbit derived unless otherwise noted): anti-FGFR1 (#9740), phospho-FGFR (Y653/654, #3476, mouse derived), phospho-FRS2 (Y436, #3861; Y196, #3864), PLCγ (#5690), phospho-PLCγ (Y783, #2821), ERK (#4695), phospho-ERK (T202/Y204 #4370), Akt (#4691), phospho-Akt (S473 #4060), STAT3 (#4904), phospho-STAT3 (Y705, #9145), E-cadherin (#3195), Vimentin (#5741), ZEB1 (#3396), N-cadherin (#13116), HIF1α (#14179), Bcl-XL (#2764), Hsp90 (#4877), FGF2 (#61977), TGFβ (#3711), Cyclin D1 (#2978), GAPDH (#2118), horseradish peroxidase (HRP)-linked rabbit IgG secondary antibody (#7074) and HRP-linked mouse IgG secondary antibody (#7076), from Cell Signaling Technology. Anti-FRS2 (#10425, mouse derived) was purchased from Abcam. AZD4547 was purchased from Selleck Chemistry. The following CyTOF antibodies were purchased from Fluidigm Inc.: STAT3 (#3173003A), pSTAT3 (#3158005A), Vimentin (#3154014A), TGFβ (#3163010B), SOX2 (#3150019B), and Nanog (#3169014A). Custom antibody-metal conjugations were performed using kits purchased from Fluidigm, Inc. (PRD002) and carrier-free antibodies targeting FGFR1 (#9740), pFGFR1 (#3476), pFRS2 Y196 (#3864), and ZEB1 (#3396) were purchased from Cell Signaling Technology.
Ryan M.R., Sohl C.D., Luo B, & Anderson K.S. (2018). The FGFR1 V561M Gatekeeper Mutation Drives AZD4547 Resistance through STAT3 Activation and EMT. Molecular cancer research : MCR, 17(2), 532-543.
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2

Immunoblot-based Characterization of Receptor Signaling

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Immunoblots were probed using antibodies against tEGFR (Cell Signaling), pERK1/2, tERK1/2 (Cell Signaling Technology), pFGFR1, tFGFR1 (Invitrogen), tFGFR2, tFGFR3 () and tFGFR4 (Santa Cruz). pFRS2-Y436 (Abcam), pFRS2-Y196 (Cell Signaling), tFRS2 (Santa Cruz), tIR (Cell Signaling), tPLC γ (Santa Cruz), SH3BP2 (Abcam), pY (Invitrogen), and α-tubulin. Pairwise p- and t- proteins were analyzed on separate gels/immunoblots using the same protein lysate. Immunoblots were quantified using Image J software (NIH). For the co-immunoprecipitation experiments of Fig. 6G, the FGFR1 or SH3BP2 antibody was conjugated to Dynabeads (Invitrogen) overnight, added to NCI-H520 cell extract and incubated at 4°C overnight.
Lin L., Chamberlain L., Pak M.L., Nagarajan A., Gupta R., Zhu L.J., Wright C.M., Fong K.M., Wajapeyee N, & Green M.R. (2014). A Large-Scale RNAi-Based Mouse Tumorigenesis Screen Identifies New Lung Cancer Tumor Suppressors that Repress FGFR Signaling. Cancer discovery, 4(10), 1168-1181.
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