Phenotypic identification of LAB isolates was performed by API 50 CHL (API kit, bioMérieux, France) assay. Purified LAB cultures were cultivated in 20 ml MRS broth incubated at 37 °C overnight, after which they were washed and re-suspended in API®50 CHL medium (bioMerieux®SA 69280, France). The turbidity of the suspensions was determined by the McFarland method according to the instructions provided by the manufacturer. Cell suspensions were inoculated into API 50 CHL strip wells and overlaid with paraffin oil to create an anaerobic condition. The strips were incubated at 37 °C. The results were recorded after 24 h and confirmed after 48 h. A positive reaction was indicated by a yellow colour except for the esculine test (black). Colour reactions were scored against a chart provided by the manufacture (De Angelis et al., 2001 (link)). The results were analysed with API WEB (bioMerieux) database version 5.0.
Api 50 chl api kit
Manufactured by bioMérieux
Sourced in France
The API 50 CHL is a standardized identification system for Lactobacillus and Bifidobacterium species. It is a biochemical test kit used in clinical and food microbiology laboratories to identify and differentiate various species of Lactobacillus and Bifidobacterium bacteria.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using api 50 chl api kit
1
Phenotypic Identification of Lactic Acid Bacteria
2
Phenotypic Identification of Lactic Acid Bacteria using API 50 CHL
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Phenotypic identi cation of LAB isolates using API 50 CHL kit Phenotypic identi cation of LAB isolates was performed by API 50 CHL (API kit, bioMérieux, France) assay. Puri ed LAB cultures were cultivated in 20 ml MRS broth incubated at 37°C overnight, after which they were washed and re-suspended in API ® 50 CHL medium (bioMerieux ® SA 69280, France). The turbidity of the suspensions was determined by the McFarland method according to the instructions provided by the manufacturer. Cell suspensions were transferred into API 50 CHL strip wells and overlaid with para n oil to create an anaerobic condition. The strips were incubated at 37°C. The results were read after 24 h and con rmed after 48 h. Fermentation of carbohydrates was indicated by a yellow color except for the esculine test (black). Color reactions were scored against a chart provided by the manufacture [20] (link). The results were analyzed with API WEB (bioMerieux) database version 5.0.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!