Antibodies used in this study were as follows: rabbit anti-phospho-STING (D8F4W, dilution 1:1000 for western blot), rabbit anti-phospho-STING (D1C4T, dilution 1:1000 for immunofluorescence), rabbit anti-phospho-TBK1 (D52C2, dilution 1:1000), rabbit anti-IRF3 (D83B9, dilution 1:1000), rabbit anti-phospho-IRF3 (4D4G, dilution 1:1000) (Cell Signaling Technology); rabbit anti-TBK1 (ab40676, dilution 1:1000) (Abcam); mouse anti-α-tubulin (DM1A, dilution 1:1000) (Sigma-Aldrich); Goat Anti-Rabbit IgG (H + L) Mouse/Human ads-HRP (4050-05, dilution 1:10,000) and Goat Anti-Mouse IgG (H + L) Human ads-HRP (1031-05, dilution 1:10,000) (Southern Biotech); sheep anti-TGN38 (AHP499G, dilution 1:200) (Bio-Rad); mouse anti-GM130 (610823, dilution 1:2000) (BD Biosciences). The antibody against STING was generated by immunizing rabbit with recombinant glutathione S-transferase (GST)-hSTING-C (amino acids 173-379) produced in E. coli.
Kemmoku H., Kuchitsu Y., Mukai K., & Taguchi T. (2021). Kinase activity of TBK1 is required for its binding to STING, but not for its recruitment to the Golgi.
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