Peptone dextrose agar

Every 6-hour of fermentation, the inoculated lamtoro seed was sampled for lactic acid bacteria, yeast, and mold enumeration. Approximately 1 g of each sample was crushed and homogenized in 9 mL of 0.85% NaCl solution then each sample was serially diluted using sterile 0.85% NaCl solution.
For Lactic acid bacteria (LAB) enumeration, 1 mL sample was pour plating on de Man Ragosa Sharpe Agar (Merck) supplemented with 1 % CaCO 3 and 0.02 ppm sodium azide then incubated 37 o C for 48 hrs (Nudyanto dan Zubaidah, 2015) . For yeast enumeration, 0.1 mL sample was spread plating on Malt Extract Agar (Merck) supplemented with 200 ppm chloramphenicol and 0.5 % calcium propionate then incubated 28°C for 48 hrs (Ebabhi et al., 2013) . For mold enumeration, 0.1 mL sample was spread plating on Peptone Dextrose Agar (Merck) supplemented with 200 ppm chloramphenicol and 0.1% tartaric acid then incubated 30 o C for 48 hrs (Da Silva et al., 2013) . The total number of LAB, yeast and mold colonies was expressed as the number of colonies per mL (CFU/mL) sample.

The samples were taken immediately after lamtoro seed has been inoculated with laru (0 hr) and every 12 hrs during continued fermentation begin from 42 nd hrs fermentation until 138 th hrs. Samples were collected from 3 times lamtoro tempe production. Approximately 1 g of each sample was crushed and homogenized in 9 mL of 0.85% NaCl solution then each sample was serially diluted using sterile 0.85% NaCl solution.
For lactic acid bacteria (LAB) enumeration, 1 mL of suspension from 10 -5 -10 -7 dilution was pour-plated with de Man Ragosa Sharpe Agar (Merck) supplemented with 1% CaCO 3 and 0.02 ppm sodium azide then incubated 37 o C for 48 hrs (Nudyanto dan Zubaidah, 2015) . While for yeast enumeration, 0.1 mL suspension from 10 -2 -10 -4 dilution was spread-plated on Malt Extract Agar (Merck) supplemented with 200 ppm chloramphenicol and 0.5% calcium propionate then incubated 28 o C for 48 hrs (Ebabhi et al., 2013) . Whereas mold enumeration was conducted by spread plating 0.1 mL suspension from 10 -1 -10 -3 dilution on Peptone Dextrose Agar (Merck) supplemented with 200 ppm chloramphenicol and 0.1% tartaric acid then incubated 30 o C for 48 hrs (Da Silva et al., 2013) . All plating for microbial analysis was done in two replicates and the total number of LAB, yeast and mold colonies were expressed as the number of colonies per mL (CFU/mL) sample.