Loricrin prb 145p

Manufactured by Fortrea

Loricrin (PRB-145P) is a laboratory equipment product manufactured by Fortrea. It is a device used for the analysis and detection of specific proteins or molecules in a sample. The core function of Loricrin is to provide accurate and reliable results for research and scientific applications.

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Lab products found in correlation

Actin, by Merck Group (2 mentions) Phospho histone h3, by Cell Signaling Technology (1 mentions) Anti mouse igg alexa594, by Thermo Fisher Scientific (1 mentions) Alexa 594 anti rabbit igg, by Thermo Fisher Scientific (1 mentions) γh2ax, by Merck Group (1 mentions) Deadend fluorometric tunel system, by Promega (1 mentions) Involucrin, by Santa Cruz Biotechnology (1 mentions) Desmocollin 1, by Santa Cruz Biotechnology (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Secondary biotin conjugated anti rabbit or anti mouse antibodies, by Jackson ImmunoResearch (1 mentions) Anti turbogfp, by Evrogen (1 mentions) Lsm 510 meta confocal microscope, by Zeiss (1 mentions) Padi3, by Abcam (1 mentions) Anti flag m2 f3165, by Merck Group (1 mentions) C myc, by Cell Signaling Technology (1 mentions)

4 protocols using loricrin prb 145p

Histological and Immunohistochemical Analysis of Skin Samples

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Skin samples were embedded in paraffin and cut in 5-μm sections. In order to evaluate basic histopathological features, the sections were stained with haematoxylin and eosin and examined under a microscope. Several immunohistochemical markers were examined at different time points as described in the figure legends. The following antibodies were used: K14 (MS115; Thermoscientific), Loricrin (PRB-145P; Covance), γH2AX (05-636; Millipore), p53 (NCL-p53-CM5p; Leica), Phospho-Histone H3 (9701; Cell signalling), F4/80 (MCAP497; Serotec), Ly6G (551459; BD), anti-Mouse IgG-Alexa594 (A11005; Invitrogen), anti-Rabbit IgG-Alexa594 (A11012; Invitrogen), anti-Rabbit IgG-Biotin (NEF813; Perkin Elmer), anti-Mouse IgG-Biotin (BA-9200; Biozol) and anti-Rat IgG-Biotin (E0468; Dako). TdT-mediated dUTP nick end labelling assay was performed using DeadEnd™ Fluorometric TUNEL System (G3250; Promega).

Kim C, & Pasparakis M. (2014). Epidermal p65/NF-κB signalling is essential for skin carcinogenesis. EMBO Molecular Medicine, 6(7), 970-983.

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Antibody Panel for Western Blot and IHC

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Antibodies used for Western blot analysis and immunohistochemistry were: Actin (A5441; Sigma-Aldrich), Flag (F7425; Sigma-Aldrich); Desmocollin-1 (sc18115; Santa Cruz Biotechnology, Inc.); Desmoglein-1 (sc20114; Santa Cruz Biotechnology, Inc.); Flg (PRB-417P; Covance); KLK5 (ab7283; Abcam); Involucrin (sc15230; Santa Cruz Biotechnology, Inc.); Loricrin (PRB-145P; Covance); TSLP (AF555; R&D Systems); and CD3 (ab5690; Abcam).

Furio L., de Veer S., Jaillet M., Briot A., Robin A., Deraison C, & Hovnanian A. (2014). Transgenic kallikrein 5 mice reproduce major cutaneous and systemic hallmarks of Netherton syndrome. The Journal of Experimental Medicine, 211(3), 499-513.

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Protein Analysis of SMAD Signaling

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Polyclonal antibodies against pSMAD2/3 Ser 465/467 (3108 P), SMAD2 (5339 P), and SMAD3 (9523 P) were from Cell Signaling Technology, Inc. Actin (A2066, Sigma-Aldrich, St. Louis, MO) was used as protein loading control. Secondary peroxidase-conjugated anti-rabbit antibodies were from GE HealthCare. The GILZ antibody was purchased from eBioscience (14-4033).
For immunostaining, rabbit polyclonal antibodies against K6 (PRB-169P) and loricrin (PRB-145P) were from Covance, Berkeley, CA, and Caspase-14 (sc-5628) and p-SMAD2/3 (sc-11769-R) were from Santa Cruz, CA. Secondary biotin-conjugated anti-rabbit or anti-mouse antibodies (Jackson ImmunoResearch, West Grove, PA) were used.

Carceller E., Ballegeer M., Deckers J., Riccardi C., Bruscoli S., Hochepied T., Libert C, & Pérez P. (2016). Overexpression of Glucocorticoid-induced Leucine Zipper (GILZ) increases susceptibility to Imiquimod-induced psoriasis and involves cutaneous activation of TGF-β1. Scientific Reports, 6, 38825.

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Fluorescence Imaging of Cellular Proteins

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We used the following antibodies: anti-Flag m2 (F3165) from Sigma-Aldrich (St. Louis, MO); anti-turboGFP from Evrogen (Moscow, Russia); loricrin (PRB-145P) from Covance (Emeryville, CA); c-Myc (#2276) from Cell Signaling (Danvers, MA); PADi3 from Abcam (ab183209, Cambridge, United Kingdom) and LifeSpan BioSciences (LS-C153534, Seattle, WA); and TGM1 (GTX102652) from GeneTex (Irvine, CA). Polyclonal rabbit anti-murine ZDHHC13 antibody was raised by Yao-Hong Biotechnology Inc (New Taipei City, Taiwan) (Supplementary Figure S1).
The secondary antibodies coupled to Alexa 488 were purchased from Invitrogen (Carlsbad, CA), and the avidin and biotin complex kit and secondary antibody were obtained from Vector Labs (Burlingame, CA). For immunofluorescence staining, nuclei were counterstained with DAPI reagents. Fluorescence images were analyzed using a Zeiss LSM 510 meta-confocal microscope (Carl Zeiss, Oberkochen, Germany).

Chen L.Y., Lin K.R., Chen Y.J., Chiang Y.J., Ho K.C., Shen L.F., Song I.W., Liu K.M., Yang-Yen H.F., Chen Y.J., Chen Y.T., Liu F.T, & Yen J.J.Y. (2020). Palmitoyl Acyltransferase Activity of ZDHHC13 Regulates Skin Barrier Development Partly by Controlling PADi3 and TGM1 Protein Stability. The Journal of investigative dermatology, 140(5).

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