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4 protocols using 2 mercaptosuccinic acid

1

Synthesis of Gold Nanoparticles

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Materials Allylamine (AA) (98%, Aldrich), hydrogen tetrachloroaurate (99.9985%, ProSciTech), trisodium citrate (99%, BHD Chemicals, Australia Pty. Ltd), 2-mercaptosuccinic acid (97%, Aldrich), were used as received.
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2

Synthesis of Polymer Composite Materials

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Allylamine (AA) (98%, Aldrich), acrylic acid (AAC) (99%, Aldrich), octadiene (OD) (98%, Aldrich), hydrogen tetrachloroaurate (99.9985%, ProSciTech), trisodium citrate (99%, BHD Chemicals, Australia Pty. Ltd.), 2-mercaptosuccinic acid (97%, Aldrich), Poly (Styrene Sulphonate) (Aldrich) were used as received.
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3

Synthesis and Characterization of Functionalized Nanoparticles

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2-Methyl-2-oxazoline, NaOH pellets, phosphate buffer saline (PBS) tablets, foetal bovine serum (FBS), 2-mercaptosuccinic acid (MSA) (97%), sodium borohydride (NaBH4), nitric acid (70%) were purchased from Sigma-Aldrich Australia and used as received. Hydrochloric acid (36%, Ajax Finechem Pty. Ltd. Australia) was used as received. Dulbecco's Modified Eagle Medium (DMEM) was purchased from Gibco. Penicillin, streptomycin, l-glutamine, MEM non-essential amino acids, cell counting kit-8 (CCK-8), Naphthol AS-MX phosphate and Fast Red TR were obtained from Sigma. Silver nitrate (AgNO3) and glass coverslips were bought from ProSciTech, Australia. Ultra-pure MilliQ water (resistivity 18.2 Ω) was used for all experiments and cleaning procedures.
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4

Silica-based Hybrid Materials Synthesis

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TEOS (98%), vinyltrimethoxysilane (VTMS,
98%), mercaptoacetic acid (MAA, 97%), 2-mercaptosuccinic acid (MSA,
97%), benzophenone (Ph2CO, 99%), Brij 58, and Pluronic
F127 were purchased from Sigma-Aldrich and used as received. Hydrochloric
acid (37%), Pb(NO3)2, and methanol were purchased
from Merck. methanol, pure grade ethanol, and Milli-Q water were used
as solvents. methanol was dried over activated MS-3 Å before
use.
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