HT-29 colon carcinoma cells were purchased from American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% (v/v) fetal bovine serum (FBS) at 37 °C in a 5% (v/v) CO2 incubator. Primary human umbilical vein vascular endothelial cells (HUVECs), kindly provided by Professor Li Jing (State Key Laboratory of Reproductive Medicine, Nanjing Medical University, China), and cultured in ECM complete medium (ScienCell, San Diego, CA, USA) at 37 °C in a 5% (v/v) CO2 incubator. PBMCs were isolated from leukapheresis products, kindly provided by the Nanjing Red Cross Blood Center (Nanjing, China).
Ht 29 colon carcinoma cells
Manufactured by American Type Culture Collection
Sourced in United States
HT-29 cells are a human colon adenocarcinoma cell line derived from a 44-year-old Caucasian female. These cells are widely used in cancer research to study colon cancer pathogenesis and evaluate potential therapeutic agents.
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Lab products found in correlation
2 protocols using ht 29 colon carcinoma cells
1
Culturing HT-29, HUVEC, and PBMCs
2
Cell Lines for Glioma and Meningioma Research
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The malignant human glioma cell lines (U-87, LN-229, U-251, LN-308) were kindly provided by Professor N. de Tribolet (Lausanne, Switzerland). Tu-2449 is a glioma cell line derived from a spontaneous tumor in GFAP-v-src-transgenic mice and was provided by J. Weissenberger (Frankfurt, Germany) (34 (link)). HT-29 colon carcinoma cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). The malignant meningioma cell line IOMM-Lee was kindly provided by David H. Gutmann (Department of Neurology, Washington University School of Medicine, St. Louis, MO, USA) (35 (link)–39 (link)). Mouse brain endothelial cells bEnd.3 immortalized with the polyoma virus middle T oncogene were kindly provided by Werner Risau (Max-Planck Institute for Physiological and Clinical Research, Bad Nauheim, Germany) (40 (link)–42 (link)). All cells were maintained in DMEM containing 10% fetal calf serum, 2 mM glutamine and penicillin (100 IU/ml)/streptomycin (100 µg/ml) (43 (link)).
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