Daporinad (99.94%) was purchased from MedChem Express (Monmouth Junction, NJ, USA). Verapamil, glutathione (GSH), uridine-50-diphosphoglucuronic acid triammonium salt (UDPGA) were purchased from Sigma-Aldrich (Yong-in, Gyeonggi, Korea). Dimethyl sulfoxide (DMSO), formic acid (FA) and methanol (MeOH) were obtained from Daejung reagents (Siheung, Gyeonggi, Korea). HPLC grade acetonitrile (ACN) and distilled water (DW) were from Samchun chemical (Pyeongtaek, Gyeonggi, Korea).
ICR mouse and human liver microsomes, β-nicotinamide adenine dinucleotide hydrate (NADPH) regenerating system solution A and B were from Corning (Tewksbury, MA, USA) and TiCl3 was bought from Kanto chemical (Tokyo, Japan). All other chemicals and reagents were analytical grade and purchased from commercial sources.
ICR mouse and human liver microsomes, β-nicotinamide adenine dinucleotide hydrate (NADPH) regenerating system solution A and B were from Corning (Tewksbury, MA, USA) and TiCl3 was bought from Kanto chemical (Tokyo, Japan). All other chemicals and reagents were analytical grade and purchased from commercial sources.