Snu 387 cell line

Human HCC cell lines (HCCLM3, HuH-7 and SNU-387) and normal human liver epithelial cell line (THLE-3) were selected for this study. HCCLM3, HuH-7 and THLE-3 cells obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Thermo Fisher Scientific, Inc.). SNU-387 cell line purchased from American Type Culture Collection (ATCC; Manassas, VA, USA) was inoculated in Roswell Park Memorial Institute (RPMI)-1640 Medium (HyClone, South Logan, UT, USA). All mediums were supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics and kept under the condition of 5% CO₂ and 37°C.

HepG2 and Hep3B cell lines were purchased from American Type Culture Collection and cultured in Eagle’s Minimum Essential Medium with 10% fetal bovine serum (FBS), 1% GlutaMAX-I and 100 U mL⁻¹ penicillin-streptomycin (Thermo Fisher Scientific) in a humidified incubator with 5% CO₂. SNU387 cell line was purchased from American Type Culture Collection and cultured in RPMI-1640 Medium with 10% FBS, 1% GlutaMAX-I and 100 U mL⁻¹ penicillin-streptomycin in a humidified incubator with 5% CO₂.