Log In Sign up
The largest database of trusted experimental protocols

Np1 rsa

Manufactured by LGC
Visit Supplier

The NP1-RSA is a laboratory instrument designed for the detection and analysis of nucleic acids. It utilizes real-time PCR technology to amplify and quantify specific genetic sequences. The core function of the NP1-RSA is to provide accurate and reliable data for research, diagnostics, and other applications requiring nucleic acid analysis.

Automatically generated - may contain errors

Lab products found in correlation

Hel antigen, by Merck Group (2 mentions) Hrp labeled anti igg1 antibodies, by Southern Biotech (2 mentions) Ovalbumin (ova), by Merck Group (2 mentions) Spectramax m5, by Molecular Devices (2 mentions) Bovine serum albumin (bsa), by Research Products International (2 mentions) Np25 bsa, by LGC (2 mentions) Np10 bsa, by LGC (2 mentions)

2 protocols using np1 rsa

1

Evaluation of Antigen-Specific IgG1 Antibodies

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum was harvested from blood collected by lateral tail vein sampling or cardiac puncture postmortem. OVA-specific and NP-specific IgG1 ELISA was performed as described (Brooks et al., 2020 (link); Tan et al., 2020 (link)). Briefly, ELISA plates were coated with NP conjugates (NP1-RSA, NP25-BSA, NP10-BSA all from LGC Biosearch; NP3-OVA, NP7-OVA, NP9-OVA and NP19-OVA were conjugated in-house as described above), OVA (Sigma Aldrich), BSA (Research Products International) or HEL antigen (10μg/mL; Sigma Aldrich), samples were added, and HRP-labeled anti-IgG1 antibodies (Southern Biotech) were used to detect plate-bound IgG1. Plates were developed with slow kinetic form TMB (Sigma Aldrich) and stopped with 1N sulfuric acid. Absorbance was measured at 450nm using spectrophotometer (SpectraMax M5, Molecular Devices). Relative titers were interpolated from standard curves generated using samples with known high titers of antigen-specific antibody.
Brooks J.F., Tan C., Mueller J.L., Hibiya K., Hiwa R., Vykunta V, & Zikherman J. (2021). Negative feedback by NUR77/Nr4a1 restrains B cell clonal dominance during early T-dependent immune responses. Cell reports, 36(9), 109645.
+ Open protocol
+ Expand
2

Evaluation of Antigen-Specific IgG1 Antibodies

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum was harvested from blood collected by lateral tail vein sampling or cardiac puncture postmortem. OVA-specific and NP-specific IgG1 ELISA was performed as described (Brooks et al., 2020 (link); Tan et al., 2020 (link)). Briefly, ELISA plates were coated with NP conjugates (NP1-RSA, NP25-BSA, NP10-BSA all from LGC Biosearch; NP3-OVA, NP7-OVA, NP9-OVA and NP19-OVA were conjugated in-house as described above), OVA (Sigma Aldrich), BSA (Research Products International) or HEL antigen (10μg/mL; Sigma Aldrich), samples were added, and HRP-labeled anti-IgG1 antibodies (Southern Biotech) were used to detect plate-bound IgG1. Plates were developed with slow kinetic form TMB (Sigma Aldrich) and stopped with 1N sulfuric acid. Absorbance was measured at 450nm using spectrophotometer (SpectraMax M5, Molecular Devices). Relative titers were interpolated from standard curves generated using samples with known high titers of antigen-specific antibody.
Brooks J.F., Tan C., Mueller J.L., Hibiya K., Hiwa R., Vykunta V, & Zikherman J. (2021). Negative feedback by NUR77/Nr4a1 restrains B cell clonal dominance during early T-dependent immune responses. Cell reports, 36(9), 109645.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!