2 methoxy polyethyleneoxy propyl trimethoxysilane

Manufactured by Gelest

Sourced in Germany

2-[methoxy(polyethyleneoxy)propyl]trimethoxysilane is a silane compound with a polyethylene glycol (PEG) moiety. It has a molecular formula of C13H30O5Si and is used as a coupling agent and surfactant in various applications.

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2-[Methoxy(polyethyleneoxy)propyl]trimethoxysilane (PEG-silane) (3 citations) 2-[Methoxy(polyethyleneoxy)propyl]-trimethoxysilane (PEOS), (2 citations) 2-[Methoxy(polyethyleneoxy)-propyl]9–12-trimethoxysilane (PEG-silane) (2 citations)

8 protocols using 2 methoxy polyethyleneoxy propyl trimethoxysilane

Synthesis of Fluorescent Silica Nanoparticles

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Gadolinium (III) chloride hexahydrate (GdCl₃·6H₂O), cetyltrimethylammonium bromide (CTAB, CH₃(CH₂)₁₅N(CH₃)₃Br), diethylene glycol, tetraethoxysilane (TEOS), and (3-aminopropyl)trimethoxysilane (APTMS) were purchased from Alfa Aesar (Ward Hill, MA). Sodium hydroxide (NaOH) was purchased from VWR (Radnor, PA). Methanol, dimethyl sulfoxide (DMSO), and toluene were purchased from Fisher Scientific (Pittsburgh, PA). Tetramethylrhodamine isothiocyanate (TRITC) was purchased from Sigma-Aldrich (St. Louis, MO). 2-[Methoxy(polyethyleneoxy)propyl]trimethoxysilane was purchased from Gelest (Morrisville, PA). Dulbecco’s modified eagle medium (DMEM), Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS), and penicillin–streptomycin (pen-strep) were purchased from Life Technologies (Grand Island, NY).

Sweeney S.K., Luo Y., O’Donnell M.A, & Assouline J. (2016). Nanotechnology and cancer: improving real-time monitoring and staging of bladder cancer with multimodal mesoporous silica nanoparticles. Cancer Nanotechnology, 7, 3.

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PEGylated Coverslips for Microtubule Attachment

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PEGylated coverslips were made using methods adapted from (Gestaut et al., 2008 (link)). Glass cover slides (Fisher Scientific 12–545 M) were plasma cleaned for 5 min and transferred to glass Coplin jars containing 1 M KOH and then placed in a sonicating water bath for 20 min. Slides were rinsed thoroughly with nanopure water, then 95% ethanol and dried using a nitrogen stream. Slides were then placed in glass Coplin jars containing 1.73% 2-methoxy(polyethyleneoxy)propyltrimethoxysilane (Gelest, Inc SIM6492.7–25 g) and 0.62% n-butylamine (Acros Organics 109-73-9) in anhydrous toluene (Sigma-Aldrich 244511), prepared with glass pipettes. Coplin jars containing slides were then placed in plastic bags, purged with nitrogen and incubated for 1.5 hr at room temperature. Following this incubation, the slides were dipped successively in two beakers containing anhydrous toluene and dried using a nitrogen stream. The slides were immediately made into flow chambers, placed in 50 ml tubes and stored at −20°C. This procedure produces a PEGylated slide surface that contains small gaps for the purpose of microtubule attachment.

Sladewski T.E., Billington N., Ali M.Y., Bookwalter C.S., Lu H., Krementsova E.B., Schroer T.A, & Trybus K.M. (2018). Recruitment of two dyneins to an mRNA-dependent Bicaudal D transport complex. eLife, 7, e36306.

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Comprehensive Fluorescent Probe Preparation

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Pluronic L121, Pluronic L61, glycerol, sucrose, KCl, MgCl₂, CaCl₂, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES > 99.5%), 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (HPTS > 96%), carboxyfluorescein, sulforhodamine B, rhodamine 6 G, eriochrome black T, polyethylene glycol (PEG, MW 6000 Da), poly(vinyl alcohol) (PVA, MW 13000–23000 Da, 87–89% hydrolyzed), poly(allylamine hydrochloride) (MW 17500 Da), FITC, NHS-Fluorescein, ATP disodium salt, ADP sodium salt, valinomycin (>98%), pyruvate kinase from rabbit muscle (Type III, 350–600 units mg⁻¹ protein), lactic dehydrogenase from rabbit muscle (Type XI, 600-1,200 units mg⁻¹ protein), Bovine Serum Albumin (BSA, Product No. A7030), Bradford Reagent (Product No. B6916), NADH (reduced disodium salt, Grade II), DL-dithiothreitol (DTT > 98.0% for molecular biology), chloroform, cyclohexane, n-octadecyltrimethoxysilane, HCl (36.5–38.0%), NaOH (>98.0%, ACS reagent) were purchased from Sigma Aldrich. 2-[methoxy(polyethyleneoxy)propyl]trimethoxy silane was purchased from Gelest. Phosphoenolpyruvate was purchased from Roche. Actin (>99%, rabbit muscle) was purchased from Cytoskeleton. Pluronic F-68 (Poloxamer P188), Atto 633 NHS ester, Atto 590, and Alexa Fluor 488 conjugated phalloidin were purchased from ThermoFisher. Poly(butadiene)-b-poly(ethylene glycol) (PB-PEO) was purchased from PolymerScience. (Canada).

Seo H, & Lee H. (2022). Spatiotemporal control of signal-driven enzymatic reaction in artificial cell-like polymersomes. Nature Communications, 13, 5179.

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Capillary Microfluidic Device Construction

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A capillary microfluidic device is designed to have one tapered cylindrical capillary assembled in another cylindrical capillary with a bigger diameter. The inner cylindrical capillary is tapered by puller (P-2000, Sutter Instrument) and then carefully sanded to have 200 μm orifice. Afterward, the capillary is merged into 2-[methoxy(polyethyleneoxy)propyl] trimethoxy silane (Gelest, Inc.) for 30 min in order to make it hydrophilic, then the capillary is dried by blowing air. Finally, one tip of the bigger capillary and the junction of two capillaries are sealed into two needles (the needles were firstly cut out appropriate holes by blade so that capillaries can stuck in it) by glue.

Shang S., Zhang K., Hu H., Sun X., Liu J., Ni Y, & Zhu P. (2022). Magnetic field responsive microspheres with tunable structural colors by controlled assembly of nanoparticles. RSC Advances, 12(9), 5656-5664.

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Peptide Functionalization of Microcantilever Arrays

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Microcantilever arrays (Concentris GmbH – Switzerland) of eight gold-coated cantilevers (500 μm long, 100 μm wide and 1 μm thick) were used in the experiments. The apex – top gold surfaces of the cantilevers (20 nm gold thickness) were functionalized with our designed thiolated peptides following the procedure described for gold-thiol chemistry immobilization20 (link)53 (link). Briefly, cantilevers were cleaned with Piranha solution (30% H₂O₂:96% H₂SO₄, vol/vol) for 15 minutes, rinsed three times with MilliQ-water (18 MW) followed by ethanol, and dried in air. The arrays were incubated in 2-[methoxy(polyethyleneoxy)propyl]trimethoxysilane (10 mM, Gelest Inc. Frankfurt, Germany) for 20 minutes, rinsed with ethanol and dried in air in order to make the backside of the levers inert and reduce nonspecific binding to the silicon side. Subsequently the microcantilevers were coated with the peptides of interest. In order to make sure that only cantilevers tips were functionalized with the peptide, only the tips were dipped-in the peptide solution (1 mg/mL) and kept for 6 h; the process was also repeated once to ensure an adequate peptide coupling to the cantilever surface. Prior to use, the arrays were rinsed with 70% ethanol and copious amount of PBS solution to remove any physically adsorbed materials.

Etayash H., Jiang K., Azmi S., Thundat T, & Kaur K. (2015). Real-time Detection of Breast Cancer Cells Using Peptide-functionalized Microcantilever Arrays. Scientific Reports, 5, 13967.

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Microfluidic Polymersome Synthesis

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Microfluidic devices were fabricated following procedures outlined by Weitz et al. (Ho et al. 2008 (link)) Briefly, two glass capillaries (O.D. 1 mm) were tapered to diameters of approximately 20 and 200 µm using a Flaming/Browning micropipette puller (Sutter P-97). The tip of the smaller (injection) capillary was coated with n-octadecyltrimethoxysilane (Sigma-Aldrich) while the tip of the larger (collection) capillary was coated with 2-[methoxy(polyethyleneoxy)propyl]trimethoxysilane (Gelest, Inc.). The capillaries were coaxially aligned within a square glass capillary tube (O.D. 1.05 mm) with an intercapillary distance of 100 µm. Three 20-gauge syringe tips were epoxied over the square capillary termini to allow injection of each liquid phase with syringe pumps via polyethylene tubing (O.D. 1.32 mm). For polymersome synthesis, three liquid phases were prepared: An aqueous solution comprised of Lysogeny broth (LB, Becton-Dickson), an organic polymer solution containing 2.5 mg/mL of diblock copolymer poly(ethylene glycol)-b-poly(D,L-lactic acid) (mPEG-PDLLA, 5000 MW mPEG and 50,000 MW PDLLA, Polyscitech) and 1.0 mg/mL of poly(lactic acid) (PLA, 15,000 MW, Polysciences) dissolved in a 2:1 ratio of toluene : chloroform, and an aqueous solution comprised of 10 wt% poly(vinyl alcohol) (PVA, 13,000–23,000 MW, 98% hydrolyzed, Sigma-Aldrich) dissolved by heating in deionized water.

Barlow J., Gozzi K., Kelley C.P., Geilich B.M., Webster T.J., Chai Y., Sridhar S, & van de Ven A.L. (2016). High throughput microencapsulation of B. subtilis in semi-permeable biodegradable polymersomes for selenium remediation. Applied microbiology and biotechnology, 101(1), 455-464.

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Silanization of Glass Coverslips for Flow Chambers

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For silanization, glass coverslips were incubated in 100% methanol for 2 h with shaking. Methanol-washed coverslips were then plasma cleaned (Harrick Plasma Cleaner; HarrickPlasma, Ithaca, NY) for 2–5 min and incubated in a silane mixture (97% toluene [Sigma-Aldrich], 2% 2-methoxy(polyethyleneoxy)propyltrimethoxysilane [Gelest, Morrisville, PA], and 1% butylamine [Acros Organics, Morris Plains, NJ]) with flowing nitrogen gas for 90 min (Lowndes and Nelson, 2013 (link)). Coverslips were washed in toluene (Lowndes and Nelson, 2013 (link)) and then dried and cured with flowing nitrogen gas for 30 min. Norland optical adhesive (Norland Products, Cranbury, NJ) was used to construct flow chambers by adhering ARTUS shims (ARTUS, Eaglewood, NJ) to silanized glass coverslips before 15 min of UV irradiation (McVicker et al., 2011 (link)).

Stern J.L., Lessard D.V., Hoeprich G.J., Morfini G.A, & Berger C.L. (2017). Phosphoregulation of Tau modulates inhibition of kinesin-1 motility. Molecular Biology of the Cell, 28(8), 1079-1087.

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Polymer-based Nanocomposite Synthesis

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Polystyrene (PS, average M_w = 35 kg mol⁻¹), poly(methyl methacrylate) (PMMA, average M_w = 2 kg mol⁻¹), poly(ethylene glycol) (PEG, average M_w = 200 g mol⁻¹), poly(propylene carbonate) (PPC, average M_w = 50 kg mol⁻¹), poly(vinyl alcohol) (PVA, average M_w = 13–23 kg mol⁻¹, 87–89% hydrolyzed), silicone oil (viscosity 5 cSt), soybean oil, chloroform (purity > 99%) were all purchased from Sigma-Aldrich. 2[methoxy(polyethyleneoxy)propyl]trimethoxy silane was purchased from Gelest, Inc. Deionized water (Millipore Milli-Q grade) with a resistivity of 18.0 MΩ was used in all the experiments.

Yang Y., Kim J.B., Nam S.K., Zhang M., Xu J., Zhu J, & Kim S.H. (2023). Nanostructure-free crescent-shaped microparticles as full-color reflective pigments. Nature Communications, 14, 793.

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