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Bathocuproine disulfonic acid disodium salt bcs

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Bathocuproine disulfonic acid disodium salt (BCS) is a chemical compound that functions as a colorimetric reagent. It is used for the detection and quantification of copper ions in various applications.

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Lab products found in correlation

Bafilomycin a1, by Enzo Life Sciences (1 mentions) Copper 2 sulfate, by Merck Group (1 mentions) Elesclomol, by Selleck Chemicals (1 mentions) Pd98059, by Merck Group (1 mentions) Copper 2 sulphate, by Merck Group (1 mentions)

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Bathocuproine (22 citations)

2 protocols using bathocuproine disulfonic acid disodium salt bcs

1

Optimizing Copper-Mediated Cell Assays

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Copper (II) sulfate, tetraethylthiuram disulfide (disulfiram), and bathocuproine disulfonic acid disodium salt (BCS) were purchased from Sigma-Aldrich. Elesclomol was purchased from Selleckchem and bafilomycin A1 was purchased from Enzo Life Sciences. Copper (II) sulfate was dissolved in water and stored at room temperature. BCS, Elesclomol, bafilomycin A1, and disulfiram were dissolved in DMSO, aliquoted, and stored at −20°C until use. We used a HP D300 Liquid Dispenser (Tecan) for all experiments involving DMSO-dissolved compounds.
Schlecht U., Suresh S., Xu W., Aparicio A.M., Chu A., Proctor M.J., Davis R.W., Scharfe C, & St Onge R.P. (2014). A functional screen for copper homeostasis genes identifies a pharmacologically tractable cellular system. BMC Genomics, 15, 263.
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2

Quantifying Copper Levels with BCS

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Copper (II) sulphate and Bathocuproinedisulfonic acid disodium salt (BCS) were purchased from Sigma-Aldrich (Steinheim, Germany). Copper and BCS solutions were prepared in dH2O with stock concentrations of 10–20 mM and 200 mM, respectively. The solutions were filtered through 0.2 μM filters before usage. PD98059 was obtained from Sigma-Aldrich. A stock solution of PD98059 was prepared in DMSO.
Frisk J.M., Kjellén L., Kaler S.G., Pejler G, & Öhrvik H. (2017). Copper Regulates Maturation and Expression of a MITF:Tryptase Axis in Mast Cells. Journal of immunology (Baltimore, Md. : 1950), 199(12), 4132-4141.
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