Oligo primers

Total RNA was extracted from human PRLoma tissues and xenograft tumours using Trizol Reagent (Invitrogen, Carlsbad, CA, USA), according to the product manual. We reverse‐transcribed 2 μg RNA from each sample by extension of oligo primers (TaKaRa) using M‐MLV reverse transcriptase (New England Biolabs) following the manufacturer's protocol. Real‐time PCR was performed on a Bio‐Rad IQ5 cycler using a SYBR Green reaction mix (Takara). Primers are listed below. Relative expression levels were standardised to GAPDH as internal control for all real‐time PCR assays.
Hum‐ERα Forward Primer: TCTGTCTCCTGCATACACTC
Reverse Primer: GGGAATCCTCACGCTTAG
Hum‐ERβ Forward Primer: GCTTTGGTTTGGGTGATTG
Reverse Primer: CCGAGTTGATTAGAGGGTC
Hum‐GAPDH Forward Primer: TGTGGGCATCAATGGATTTGG
Reverse Primer: ACACCATGTATTCCGGGTCAAT
Rat‐ERα Forward Primer:GCTCCTAACTTGCTCTTGG
Reverse Primer:GGACTCGGTGGATGTGGT
Rat‐ERβ Forward Primer:TCTCCTTTAGCGACCCA
Reverse Primer:ACGCCGTAATGATACCC
Rat‐GAPDH Forward Primer:TTCTTGTGCAGTGCCAGCCTCGTC
Reverse Primer:TAGGAACACGGAAGGCCATGCCAG

We randomly collected 52 human prostate tissue samples from surgery of PCa patients and extracted total RNA using TransZol Up Plus Kit (Transgen Biotech, Beijing, China). After the total RNA was converted to cDNA using random hexamers, oligo primers and reverse transcriptase (Takara), the MTR mRNA were quantified by real-time PCR using the ABI Prism 7900 sequence detection system with GAPDH as an internal control. Each reaction was performed in triplicate. The primers utilized are listed in supplementary Table 6.