Ldc1267

Manufactured by Selleck Chemicals

Sourced in United States

The LDC1267 is a laboratory device designed for liquid chromatography applications. It is a high-performance liquid chromatography (HPLC) detector that measures the absorbance of analytes in a liquid sample as they elute from a chromatographic column. The LDC1267 provides precise and reliable measurements to support analytical chemistry procedures.

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Lab products found in correlation

Survivin, by Santa Cruz Biotechnology (1 mentions) P akt ser473, by Cell Signaling Technology (1 mentions) Erk1 2, by R&D Systems (1 mentions) Cip2a, by Santa Cruz Biotechnology (1 mentions) P axl y779, by R&D Systems (1 mentions) Anti pp2a, by Cell Signaling Technology (1 mentions) Bsa a1470, by Merck Group (1 mentions) Torin1, by Selleck Chemicals (1 mentions) Bsa alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Dq green bsa, by Thermo Fisher Scientific (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions)

2 protocols using ldc1267

AXL-Fc Receptor Inhibition Assay

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AXL-Fc protein fragment (AXL-Fc) was purchased from R&D Systems (St. Paul, MN, USA). LDC1267 (a TAM family inhibitor) was purchased from Selleck (Houston, TX, USA). Rat GAS6 recombinant protein (RPA204Ra01) was purchased from Cloud-clone Corp. Antibodies for immunoblotting, including DAPK, p-DAPK (S308), CIP2A, AKT, MYC, Survivin and Actin, were purchased from Santa Cruz Biotechnology (San Diego, CA). ERK1/2, p-ERK1 (T202/Y204) / ERK2 (T185/Y187), and p-AXL (Y779) were purchased from R&D Systems (St. Paul, MN, USA). Other antibodies, including anti-PP2A and p-AKT (Ser473), were purchased from Cell Signaling (Danvers, MA).

Chen Y.L., Tsai Y.T., Chao T.T., Wu Y.N., Chen M.C., Lin Y.H., Liao C.H., Chou S.S, & Chiang H.S. (2018). DAPK and CIP2A are involved in GAS6/AXL-mediated Schwann cell proliferation in a rat model of bilateral cavernous nerve injury. Oncotarget, 9(5), 6402-6415.

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Immunoblotting Assay for Cell Signaling

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Antibodies to Akt (2920), Akt pSer473 (4060), Akt pThr308 (13038), S6K1 pThr389 (9234), S6K (2708), S6 pSer235/236 (4856), S6 (2317), Erk1/2 pThr202/Tyr204 (4377), Erk1/2 (9107), Lats1 pT1079 (8654), Lats1 (3477), Nf2 (6995), Lamin A/C (4777), Histone H3 (3638), pan-TEAD (13295), Taz (4883), and human Axl (8661) were purchased from Cell Signaling Technology. Antibodies to Yap (63.7) and Ankrd1 (G-2) were purchased from Santa Cruz Biotechnology. Antibodies to Vinculin (V9131), Actin (A5441), and Gapdh (G8795) were purchased from Sigma-Aldrich. Antibodies to mouse Axl (AF854) and recombinant human Gas6 (895-GSB-050) were from R&D Systems. Secondary antibodies were from GE Healthcare (HRP-linked anti-rabbit and HRP-linked anti-mouse) and Thermo Fisher Scientific (HRP-linked anti-goat).
Alexa fluor 647 BSA, Oregon Green dextran (70 kDA), DQ Green BSA, and Hoechst 33342 were from Life Technologies. DAPI was from Sigma-Alrich. Inhibitors were purchased from Cayman Chemical (GDC-0941), Tocris Bioscience (Torin1), and Selleck Chemicals (LDC1267). Amino acid starvation media were from Athena (DMEM/F12 0423) or prepared by the Memorial Sloan Kettering Cancer Center Media Core Facility. Dialyzed FBS was from Gemini Biosystems and BSA (A1470) was from Sigma.

King B., Araki J., Palm W, & Thompson C.B. (2020). Yap/Taz promote the scavenging of extracellular nutrients through macropinocytosis. Genes & Development, 34(19-20), 1345-1358.

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