Ludox TMA colloidal silica (Sigma-Aldrich) was used as the adsorbent in this study. The Ludox dispersion was dialyzed for 5 days against DI water (water changed 3 times per day) to remove remaining salt. Its mean particle diameter
D was 21 nm (determined by dynamic light scattering). Its specific surface area
as was 128 m
2/g (value from the manufacturer), in agreement with the geometric surface area derived from the particle diameter,
ageom = 6/
ρsD = 130 m
2/g, based on a mass density of silica
ρs of 2.20 g/cm
3. The value of
as/
ageom = 1.02 indicates a low surface roughness of the particles [32 (
link)]. The electrophoretic mobility of the Ludox particles was determined by electrophoretic light scattering of a 1 wt% dispersion as described elsewhere [2 (
link)], using a
Nano Zetasizer (Malvern Instruments, UK). Three measurements, each consisting of at least 50 runs, were performed for each sample.
Lysozyme from chicken egg white lyophilized powder (Sigma-Aldrich, ≥40,000 units/mg protein, lot SLBH9534V, purity ≥90 %) and ß-lactoglobulin from bovine milk (Sigma-Aldrich, lot SLBC4958V, purity ≥90 %) were used in this study.
Meissner J., Prause A., Bharti B, & Findenegg G.H. (2015). Characterization of protein adsorption onto silica nanoparticles: influence of pH and ionic strength. Colloid and Polymer Science, 293(11), 3381-3391.