Crosses were set up and animals were treated as for the lifespan assay. After 50 days (for AMPK knockdowns and respective controls) or 18 days (for Trehalase knockdown and the respective control) at 29°C the flies’ heads were embedded in Epon as described in Stork et al. (2008) (link). 1 mm semi-thin sections were made using a Leica EM UC7 Ultra Microtome, stained with toluidine blue, and imaged using a Zeiss Axiophot.
Em uc7 ultra microtome
Manufactured by Zeiss
The EM UC7 Ultra Microtome is a precision instrument designed for cutting ultra-thin sections of samples for electron microscopy. It features a high-precision feed mechanism and a sturdy, vibration-free construction to ensure the accuracy and quality of the resulting sections.
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Lab products found in correlation
3 protocols using em uc7 ultra microtome
1
Imaging Lifespan-Related Brain Changes
2
Imaging Lifespan-Related Brain Changes
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Crosses were set up and animals were treated as for the lifespan assay. After 50 days (for AMPK knockdowns and respective controls) or 18 days (for Trehalase knockdown and the respective control) at 29°C the flies’ heads were embedded in Epon as described in Stork et al. (2008) (link). 1 mm semi-thin sections were made using a Leica EM UC7 Ultra Microtome, stained with toluidine blue, and imaged using a Zeiss Axiophot.
3
Quantifying Autophagosomes in VacA-Treated AGS Cells
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AGS cells were treated with 50 ng/ml VacAs1m1 toxin for 6 h, collected, and fixed in a glutaraldehyde and sodium cacodylate solution for 2 h, and then fixed with 1% OsO4 for 1 h and a half, and then stained in 3% aqueous uranyl acetate for 1 h. These cells were dehydrated with graded alcohol (50%, 70%, 80%, 90%, 95%, 100%) and embedded in Epon-Araldite resin (Canemco, #034). Ultrathin sections were prepared on a Leica EM UC7 ultramicrotome, counterstained with 0.3% lead citrate, and observed under a Zeiss EM 902A electron microscope. The autophagosome counting method was followed as described previously by Yla-Anttila et al31 (link).
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