Mice treated with or without DSS were euthanized on day 3 after the GABA treatment. For the detection of acidic mucin, AB-PAS staining of sections from paraffin-embedded tissue were evaluated using a commercial AB-PAS kit (Solarbio Life Sciences, Beijing, China) according to the manufacturer’s instructions. Sections were stained by alcian blue solution (pH 2.5) for 15 min, washed in water for 5 min, then oxidized in 1% periodic acid at room temperature for 10 min, washed in water for 5 min, and stained by Schiff’s solution for 10 min.
Ab pas kit
Manufactured by Solarbio
Sourced in China
The AB-PAS kit is a laboratory tool used for the detection and visualization of polysaccharides in biological samples. It provides a standardized method for the histochemical staining of carbohydrates, allowing for the identification and analysis of their distribution and abundance within tissues or cells.
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Lab products found in correlation
2 protocols using ab pas kit
1
Mucin Detection in Colitis Mice
2
Alcian Blue-PAS Staining Protocol
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The experiments were performed according to the instructions of the AB-PAS kit (G1285, Beijing, Solarbio Technology Co.). Sections were stained in 3% acetic acid solution with 1% Alcian blue solution (pH 2.5) for 15–20 min. The sections were then rinsed in deionized water for 2 minutes and oxidized in 0.5% periodate solution for 5 min. The slides were rinsed and stained with Schiff’s reagent for 15 min, followed by hematoxylin staining.
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