Alexa fluor 790 goat anti mouse igg

Manufactured by Jackson ImmunoResearch

Alexa Fluor 790 goat anti-mouse IgG is a secondary antibody conjugated with Alexa Fluor 790 dye. It is designed to detect and visualize mouse immunoglobulin G (IgG) in various immunoassays and imaging applications.

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Lab products found in correlation

Alexa fluor 680 goat anti rabbit igg, by Jackson ImmunoResearch (3 mentions) Alexa fluor 488 goat anti mouse igg, by Jackson ImmunoResearch (2 mentions) Alexa fluor 594 goat anti rabbit igg, by Jackson ImmunoResearch (2 mentions) Dnmt3a, by Santa Cruz Biotechnology (1 mentions) Bromodeoxyuridine (brdu), by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Gapdh, by Abmart (1 mentions) Hdac1, by ABclonal (1 mentions)

3 protocols using alexa fluor 790 goat anti mouse igg

Quantifying KCNB1 Channel Surface Expression

Cited 2 times

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Frozen aliquots of CHO-K1 cells electroporated with WT or variant KCNB1 channels were thawed and grown under the same conditions used for automated patch clamp recording. Cell-surface biotinylation and immunoblotting were performed as previously described^{7 (link),14 (link)}, 60–72h post-electroporation, using mouse anti-K_V2.1 (1:250; K89/34, Antibodies Inc), mouse anti-transferrin receptor (1:500; H68.4, ThermoFisher), and rabbit anti-calnexin (1:250; H70, Santa Cruz Biotechnology), Alexa Fluor 680-goat anti-rabbit IgG (1:20,000, Jackson ImmunoResearch) and Alexa Fluor 790-goat anti-mouse IgG (1:20,000, Jackson ImmunoResearch) antibodies.
Normalized total, surface, and surface/total protein ratio results were derived from 3 independent experiments. Calnexin immunoreactivity was present in total protein lysates and absent from the cell-surface fraction, confirming the selectivity of biotin labeling for cell-surface protein.

Kang S.K., Vanoye C.G., Misra S.N., Echevarria D.M., Calhoun J.D., O’Connor J.B., Fabre K.L., McKnight D., Demmer L., Goldenberg P., Grote L.E., Thiffault I., Saunders C., Strauss K.A., Torkamani A., van der Smagt J., van Gassen K., Carson R.P., Diaz J., Leon E., Jacher J.E., Hannibal M.C., Litwin J., Friedman N.R., Schreiber A., Lynch B., Poduri A., Marsh E.D., Goldberg E.M., Millichap J.J., George AL J.r, & Kearney J.A. (2019). Spectrum of KV2.1 Dysfunction in KCNB1-associated Neurodevelopmental Disorders. Annals of neurology, 86(6), 899-912.

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Plasmid Construction and Antibody Validation

Cited 1 time

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Plasmids pcDNA3.1-FLAG-SET8, pcDNA3.1-FLAG-UHRF1, pcDNA3.1-DNMT1, pcDNA3.1-HA-SET8, pcDNA3.1-His-Ub, pcDNA3.1-Myc-LSD1, pcDNA3.1-Myc-USP7 and pPYCAGIP-FLAG-UHRF1 were constructed in our laboratory as previously described (16 (link),38 (link),39 (link)). All mutants were generated by PCR-based point mutagenesis strategy and verified by DNA sequencing. The following antibodies were used in this study: Pan-Kme antibody (Abbkine Abm40195), UHRF1 (proteintech 21402-1-AP), monoclonal DNMT1 (homemade), monoclonal LSD1 (homemade), SET8 (Cell Signaling Technology C18B7), DNMT3A (Santa Cruz sc-20703), p53 (Santa Cruz sc-126), Ub (Santa Cruz sc-8017), GAPDH (Abmart M20006L), β-ACTIN (Sigma A5441), H3 (Abcam ab1791), H3S10P (Epitomics 1173-1), FLAG (Sigma 7425/1804), Myc (Abmart 20002 mouse), and BrdU (Sigma B8434). The following secondary antibody were used: Alexa Fluor 680 goat anti-rabbit IgG (Jackson ImmunoResearch 111-625-144), Alexa Fluor 790 goat anti-mouse IgG (Jackson ImmunoResearch 115-655-146), Alexa Fluor 594 goat anti-rabbit IgG (Jackson ImmunoResearch, 111-585-003) and Alexa Fluor 488 goat anti-mouse IgG (Jackson ImmunoResearch, 115-545-003).

Zhang H., Gao Q., Tan S., You J., Lyu C., Zhang Y., Han M., Chen Z., Li J., Wang H., Liao L., Qin J., Li J, & Wong J. (2019). SET8 prevents excessive DNA methylation by methylation-mediated degradation of UHRF1 and DNMT1. Nucleic Acids Research, 47(17), 9053-9068.

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Epigenetic Regulation Assay using Antibodies

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The commercial antibodies used in this study and their sources are as follow: histone H3 (ab1791), UBC9 (ab33044), Sin3A (ab129087), histone H3 phospho S10 (ab14955), γH2AX (ab81299), lamin B1 (ab16048) and lamin A/C (ab133256) from Abcam; SUMO-1 (ET1606-53) and SUMO-2/3 (ET1701-17) from HUABIO; H3K4me3 (07-473) from Sigma-Aldrich; HDAC1 (A0238) and HDAC2 (A2084) from ABclonal; p62 (CY9081) from Abways; RARP (#9542), Caspase-3 (#9662) from CST; FLAG, HA, and GAPDH from Abmart. RbAp46 and acetylated H3 antibodies were homemade. The following secondary antibody were used: Alexa Fluor 680 goat anti-rabbit IgG (Jackson ImmunoResearch, 111-625-144); Alexa Fluor 790 goat anti-mouse IgG (Jackson ImmunoResearch, 115-655-146); Alexa Fluor 594 goat anti-rabbit IgG (Jackson ImmunoResearch, 111-585-003); Alexa Fluor 488 goat anti-mouse IgG (Jackson ImmunoResearch, 115-545-003). ML-792 (HY-108702) was purchased from MCE.

Chen Z., Luo J., Zhang Y., Zheng S., Zhang H., Huang Y., Wong J, & Li J. (2023). SUMOylation is enriched in the nuclear matrix and required for chromosome segregation. The Journal of Biological Chemistry, 300(1), 105547.

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