Pe conjugated anti human cd14

The cells collected from differentiation cultures were analyzed by flow
cytometry (FACSCalibur, Beckton Dickinson, Franklin Lakes, NJ, USA).
PE-conjugated anti human-CD14 and FITC-conjugated anti human-CD16 Abs were
purchased from eBioscience (San Diego, California) and used at a 1:50 dilution.
The fluorescein isothiocyanate (FITC)-conjugated anti-human CD206 antibody
(Biolegend, USA), phycoerythrin (PE)-conjugated anti-human CD14 antibody
(Biolegend, USA) and PE-Cy5-conjugated anti-human CD86 antibody (Biolegend, USA)
were used following the instructions from the manufacturers. FACS data were
analyzed by FlowJo (Treestar, Ashland, OR, USA).

LPS isolated from Escherichia coli (0111:B4) was purchased from Sigma–Aldrich (St. Louis, MO, USA). Multiplex beads for: IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12 (p70), IL-17, G-CSF, GM-CSF, IFN-γ, MCP-1, MIP-1α/1β, RANTES, TNF-α, VEGF, IP-10, and GRO-α were purchased from Bio-Rad Laboratories (Hercules, CA, USA). TGFβ was purchased from Peprotech (Rocky Hill, NJ, USA) and neutralizing TGFβ Ab was purchased from R&D (Minneapolis, MN, USA). For flow cytometry PE-conjugated anti human-CD14 and FITC-conjugated anti human-CD16 Abs purchased from eBioscience (San Diego, CA, USA) were used at a 1:50 dilution.