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Stx2 chopstick electrode set

Manufactured by World Precision Instruments
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The STX2 chopstick electrode set is a laboratory equipment designed for various electrophysiology applications. It consists of a pair of slender, elongated electrodes that resemble chopsticks in appearance. The electrodes are made of durable materials and are typically used for precise positioning and recording of electrical signals in targeted areas of a sample or specimen.

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Lab products found in correlation

Evom epithelial voltohmmeter, by World Precision Instruments (1 mentions) Evom2, by World Precision Instruments (1 mentions) Epithelial volt ohm meter evom2, by World Precision Instruments (1 mentions) Evom2 epithelial voltohmmeter, by World Precision Instruments (1 mentions)

4 protocols using stx2 chopstick electrode set

1

Transepithelial Electrical Resistance Analysis

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TEER measurements were performed using an EVOM epithelial Volt/Ohm meter and STX2 chopstick electrode set (World Precision Instruments, Sarasota, FL, USA). All measurements were performed in duplicate. Final values were calculated as relative values which compare the mean measurements per condition to the mean of each baseline measurement at 0 h.
Deleu S., Arnauts K., Deprez L., Machiels K., Ferrante M., Huys G.R., Thevelein J.M., Raes J, & Vermeire S. (2023). High Acetate Concentration Protects Intestinal Barrier and Exerts Anti-Inflammatory Effects in Organoid-Derived Epithelial Monolayer Cultures from Patients with Ulcerative Colitis. International Journal of Molecular Sciences, 24(1), 768.
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2

Measuring Caco-2 Monolayer TEER Values

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The TEER-values of the Caco-2 monolayer were measured with the epithelial voltohmmeter EVOM2 (World Precision Instruments, USA) together with the STX2 Chopstick Electrode Set (World Precision Instruments, USA). First, the electrodes were cleaned with 70% isopropanol, dried, and then placed in the apical or basolateral compartment, respectively.
Schnur S., Wahl V., Metz J.K., Gillmann J., Hans F., Rotermund K., Zäh R.K., Brück D.A., Schneider M, & Hittinger M. (2022). Inflammatory bowel disease addressed by Caco-2 and monocyte-derived macrophages: an opportunity for an in vitro drug screening assay. In vitro models, 1(4-5), 365-383.
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3

Transwell Caco-2 TEER Measurement

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The TEER values of the Caco-2 cells in the Transwell® inserts were measured on a heating plate (W10, VWR, Germany) at 37 °C with the Epithelial Voltohmmeter EVOM2 (World Precision Instruments, Friedberg, Germany) and the STX2 Chopstick Electrode Set (World Precision Instruments, Friedberg, Germany). Before the measurements, the Chopstick Electrode set was cleaned in 70% 2-propanol (Th. Geyer, Renningen, Germany) for 10 min and dried at room temperature (RT).
Schnur S., Hans F., Dehne A., Osti J., Schneemann M.O., Schneider M, & Hittinger M. (2023). The Potential of Epigallocatechin-3-gallate (EGCG) as Complementary Medicine for the Treatment of Inflammatory Bowel Disease. Pharmaceuticals, 16(5), 748.
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4

Transepithelial Electrical Resistance Measurement

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Transepithelial electrical resistance [TEER] measurements were performed using an EVOM2 epithelial Volt/Ohm meter and STX2 chopstick electrode set [World Precision Instruments, Sarasota, FL, USA]. All measurements were performed in triplicate and corrected with the resistance value of an empty Transwell insert. Final values were calculated as the average resistance multiplied by the area [0.33 cm2] of the Transwell membrane and expressed as Ω.cm2.
Vancamelbeke M., Laeremans T., Vanhove W., Arnauts K., Ramalho A.S., Farré R., Cleynen I., Ferrante M, & Vermeire S. (2019). Butyrate Does Not Protect Against Inflammation-induced Loss of Epithelial Barrier Function and Cytokine Production in Primary Cell Monolayers From Patients With Ulcerative Colitis. Journal of Crohn's & Colitis, 13(10), 1351-1361.
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