Rip3 sirnas

Human BGN siRNAs, RIP3 siRNAs and non-targeting control siRNA were purchased from Shanghai GenePharma. siRNA transfection was performed as described [53 (link)].

RIP3 siRNAs were obtained from Gene-Pharma (Shanghai, China) and were transiently transfected into MDA-MB-231 cells in 6-well plates using a lipofectamine 2000 reagent kit (Invitrogen, Carlsbad, CA, USA). After 24 h of transfection, the cells were collected for Western blotting analysis as described earlier. The siRNA sequences used for experiments with human RIP3 were as follows: positive control sense, 5′-UGA CCU CAA CUA CAU GGU UTT-3′ and antisense, 5′-AAC CAU GUA GUU GAG GUC ATT-3′; negative control sense, 5′-UUC UCC GAA CGU GUC ACG UTT-3′ and antisense, 5′-ACG UGA CAC GUU CGG AGA ATT-3′; RIP3 homo-478 sense, 5′-CCG GCU CUG GUG ACU AAA UTT-3′ and antisense, 5′-AUU UAG UCA CCA GAG CCG GTT-3′; RIP3 homo-570 sense, 5′-GCU GAA AGA AGU GGU GCU UTT-3′ and antisense, 5′-AAG CAC CAC UUC UUU CAG CTT-3′; and RIP3 homo-775 sense, 5′-GAA CUG UUU GUU AAC GUA ATT-3′ and antisense, 5′-UUA CGU UAA CAA ACA GUU CTT-3′.