Agilent openlab cds chemstation software

The main compounds (>1%) of chamomile essential oil were analyzed using Agilent’s GC 7890a chromatograph (Santa Clara, CA, USA) with Agilent Open Lab CDS Chem Station software (Rev. C.01.07[27]) and FID. The analysis was conducted on two fused silica capillary columns with stationary phases: DB-5 and HP-Innowax (both 30 m × 0.25 mm, Agilent, CA, Santa Clara, USA). The carrier gas used was hydrogen, with a split ratio of 1:150 and a flow rate of 30 mL/min. The temperature program ranged from 50 to 250 °C at 2.92 °C/min, while the injector temperature was 250 °C.
The Agilent Open Lab CDS Chem Station software (Rev. C.01.07[27]) was used to identify the oil’s principal components and to compare their retention indices. The component contents (%) of the essential oil were determined by analyzing the mean retention time and peak area of four parallel chromatograms. We identified the components by comparing their DB-5 column retention indices to databases and literature data [9 (link),10 (link),11 (link),36 ].

An Agilent 1260 series HPLC instrument (Agilent, USA) equipped with a DAD detector (Agilent, USA) was used for chromatographic analysis of quantitative determination of the three marker compounds. The system control and data analysis were processed using Agilent OpenLAB CDS ChemStation software (Agilent, USA). The chromatographic separation was performed on a Waters HSS T3 (4.6 × 250 mm, 5 μm, Waters, USA) column at 40°C: (A) 0.3% phosphorous acid in water and (B) acetonitrile as mobile phases. The sample was injected (5 μL injection volume) onto the column and eluted at a flow rate of 1 mL/min according to the following gradients: initial 16% B; 0–40 min/16–18% B; 40–43 min/18–80% B; and 43–48 min/80–80% B. Ultraviolet detection was set to 330 nm.

The starch samples were debranched with isoamylase (Megazyme Ltd., Ireland). The sample preparation followed the published method with modifications [31 (link)]. The molecular structure of amylopectin was obtained by characterizing the chain-length distribution (CLD) by fluorescence-activated capillary electrophoresis (FACE) with a Laser-Induced Fluorescent detector (Agilent 7100 CE-LIF) at 488nm, and the data were analyzed using Agilent OpenLAB CDS ChemStation software (Agilent Technologies Australia Pty Ltd, Mulgrave, Victoria) [31 (link)].