Cyanine5 nhs ester cy5

PLGA (lactide:glycolide 50:50, M_w 7000–17,000), dicyclohexylcarbodiimide (DCC, 99%), N-hydroxysuccinimide (NHS, 98%), ethylenediamine (≥99.5%), PVA (87–90% hydrolyzed, MW 3000–7000), and triethylamine (≥99.5%) were obtained from Sigma-Aldrich. Cyanine5-NHS ester (Cy5, 95%) was obtained from Lumiprobe, and trehalose (D(+)-trehalose 2-hydrate) from AppliChem Panreac.

K₄[Fe(CN)₆], bovine serum albumin(BSA), MnCl₂, 2’,7’-dichlorofluorescin-diacetate (DCF-DA), Oil Red O (ORO), lipopolysaccharide (LPS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide assay kit (MAK 165) were purchased from Sigma-Aldrich (U.S.A.). 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), 2,2,6,6-Tetramethylpiperidine 1-oxyl (TEMP) and simvastatin (Sim) were purchased from Sigma-Aldrich (Shanghai). (1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG) was obtained from Corden Pharma (Switzerland). Cyanine5 NHS ester (Cy5) and cyanine7.5 NHS ester (Cy7.5) were from Lumiprobe (U.S.A.). Penicillin, streptomycin, fetal bovine serum (FBS), and Dulbecco’s Modified Eagle's Medium (DMEM) were purchased from Gibco (U.S.A.). 4’,6-Diamidino-2-phenylindole (DAPI), hematoxylin and 3,3’-dioctadecyloxacarbocyanine perchlorate (DiO) were purchased from Beyotime (China). LysoTracker Green was purchased from Invitrogen (U.S.A.). Recombinant murine interferon-γ (INF-γ) was purchased from PeproTech (U.S.A). Laser confocal scanning microscopy (CLSM)-specific dishes (35 mm × 10 mm) were purchased from Corning Inc (New York).

Yeast of Saccharomyces cerevisiae was purchased from Lesaffre International Corporation (France). Positively coated CdSe/ZnS quantum dots (QDs) with an emission wavelength at 620 nm (QD620) were obtained from Ocean NanoTech, LLC (U.S.A.). Branched polyethyleneimine (PEI) with molecular weight (M_w) of 25 kDa was purchased from Sigma (U.S.A.). Cyanine 7.5 NHS ester (Cy7.5) and Cyanine 5 NHS ester (Cy5) were supplied by Lumiprobe (U.S.A.). 4', 6-Diamidino-2-phenylindole (DAPI) was purchased from Invitrogen (U.S.A.). Cisplatin, i.e. cis-diamineplatinum (II) dichloride (CDDP) was purchased from Thermo Fisher (U.S.A.). Penicillin, streptomycin, and fetal bovine serum (FBS) were purchased from Gibco (U.S.A.). RPMI 1640 medium was obtained from HyClone (U.S.A.). All the other reagents are commercially available and used as received.