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14 protocols using omnicpicta software

1

Characterizing Antibiotic Drug Delivery by IR

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IR spectra in the 4000–400 cm−1 wavenumber range were acquired for all samples in order to assess the functional groups present and, consequently, to demonstrate the presence of the antibiotics within the drug delivery systems. A mercury cadmium telluride detector-provided Thermo Scientific Nicolet iS50 (Thermo Fischer Scientific, Waltham, MA, USA) spectrometer was employed. Measurements were performed in the attenuated total reflectance (ATR) mode. Acquisitions were made at room temperature, with a resolution of 4 cm−1 and 64 scans for each sample. The OmnicPicta software (version 8.2, Thermo Nicolet, Thermo Fischer Scientific, Waltham, MA, USA) was used for data processing.
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2

Infrared Spectroscopy of Samples

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A Thermo Scientific Nicolet iS50 (Thermo Fischer Scientific, Waltham, MA, USA) spectrometer equipped with a liquid nitrogen-cooled mercury cadmium telluride detector was used for the acquisition of IR spectra. The measurement parameters involved the attenuated total reflectance (ATR) mode, room temperature, 4 cm−1 resolution, and the range of 4000–400 cm−1. The OmnicPicta software (version 8.2, Thermo Nicolet, Thermo Fischer Scientific, Waltham, MA, USA) was used for the co-addition and processing of the 64 scans performed on each sample.
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3

Attenuated Total Reflectance FTIR Spectroscopy

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The IR spectra were acquired using a Thermo Scientific Nicolet iS50 (Thermo Fischer Scientific, Waltham, MA, USA) spectrometer with a liquid nitrogen-cooled mercury cadmium telluride detector. The measurement was performed at room temperature using the attenuated total reflectance (ATR) mode, in the range of 4000–400 cm−1 and at a resolution of 4 cm−1. For each sample, 64 scans were co-added and processed using the OmnicPicta software (version 8.2, Thermo Nicolet, Thermo Fischer Scientific, Waltham, MA, USA).
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4

Nanoparticle Characterization via FT-IR

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The synthesized nanoparticles were characterized using a Nicolet iS50FT-IR (ThermoFisher Scientific, Waltham, MA, USA) spectrometer. The measurements were performed at room temperature in the range of 4000–400 cm−1, using the resolution of 8 cm−1. All spectra were registered in attenuated total reflectance (ATR) mode using a diamond crystal. OmnicPicta software (version 8.2, Thermo Fischer Scientific, Madison, WI, USA) was used to co-add and process the 96 scans acquired for each sample.
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5

Polymer Characterization of Microplastics

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The polymer characterization of putative MPs was performed through Fourier transformed infrared (FTIR) microscopy (µ-FTIR), using a Nicolet iN10 MX Infrared Imaging Microscope (Thermo Scientific, Waltham, MA, USA). The characterization was performed in reflection mode in a wavenumber range of 4000–650 cm−1. OMNIC™ Picta software (Thermo Scientific, Waltham, MA, USA) controlled the instrument. A total of 256 scans were acquired for each spectrum, with a spectral resolution of 4 cm−1. Different libraries were used for polymer identification, including HR Aldrich Polymers, HR Coatings Technology, HR Hummel Polymer and Additives, HR Industrial Coatings, HR Polymer Additives and Plasticizers, HR Rubber Compounding Materials, HR Spectra Polymers and Plasticizers, Hummel Polymer sample Library, and Polymer Laminate Films. While all the fragments were processed under µ-FTIR, only the 25% of fibers were randomly collected from the filters and characterized because of the high number we isolated from sediment samples. The polymer composition of the other fibers was assessed through the application of the fluorophore, the 1-pyrenebutyric acid N-hydroxysuccinimidyl ester (PBN). Indeed, a recent study confirmed the use of PBN as a rapid, simple, cost-effective, and highly efficient detection method for the identification of different plastic polymers (Lee and Chae 2021 (link)).
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6

FT-IR Microscopy Analysis of Samples

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A Thermo iN10-MX Fourier transform (FT)-IR microscope (Thermo Fischer Scientific, Waltham, MA, USA) with a liquid nitrogen-cooled mercury cadmium telluride detector was used for obtaining the IR spectra. The measurement was performed in reflection mode in the range of 4000–400 cm−1 and at a resolution of 4 cm−1. 64 scans were co-added and converted to absorbance using the OmnicPicta software (Thermo Scientific) for each sample.
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7

FTIR Analysis of Cu-PS Complexes

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Spectra were obtained on an iN-10 FTIR Thermo Fisher Microscope spectrometer equipped with a narrow-band liquid-nitrogen-cooled MCTA detector. Samples of the Cu–PS complexes and the polysaccharide alone were lyophilized at −55 °C for 24 h in 96-well plates. The spectra were recorded in three areas per sample in the range of 4000 to 650 cm−1, at 2 cm−1 resolution and 64 scans; area of detection was 25 × 25 mm. The FTIR data were collected using OMNIC Picta software (Thermo Scientific OMNIC Series Software, Madison, WI, USA). Automatic baseline correction was used.
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8

Characterizing Thin Film Morphology and Composition

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AFM
amplitude micrographs were obtained in tapping mode using a Pico Plus
AFM instrument (Molecular Imaging) with aluminum-coated silicon tips
(BudgetSensors) and a spring constant of 40 N m–1. A sample area of 3 μm by 3 μm was scanned at a rate
of 1 Hz while collecting data in topographic, phase, and amplitude
modes. Morphological changes are captured in micrographs using PicoView
software (Agilent) and postprocessed using Gwyddion.30 (link)FTIR imaging was performed with a Nicolet iN10 infrared
microscope (Thermo Scientific) after mounting the optical windows
on a motorized stage for scanning the infrared map in an XY pattern. OMNIC Picta software (Thermo Scientific) was utilized for
FTIR microscopy and spectral mapping. Individual spectra corresponding
to an average of 64 scans were collected over the range of 800–4000
cm–1 with 4 cm–1 resolution. All
samples were background-subtracted using an empty optical window.
Control experiments ensured that films exposed to humid air in the
absence of O3(g) correspond to the spectral features of
catechol despite any loss by sublimation, which was carefully monitored
to remain below 5%. Data processing to obtain the CD line (or corrected
peak heights after local baseline correction) was performed10 (link) to collect kinetic data from the average of
duplicate experiments with error bars corresponding to one standard
deviation.
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9

Attenuated Total Reflectance FTIR Spectroscopy

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A Thermo Scientific Nicolet iS50 (Thermo Fischer Scientific, Waltham, MA, USA) spectrometer, with a liquid nitrogen-cooled mercury cadmium telluride detector, was used for the acquisition of the IR spectra. Measurements were performed at room temperature, in the range of 4000–400 cm−1 and at a resolution of 4 cm−1, using the attenuated total reflectance (ATR) mode. For each sample, the OmnicPicta software (version 8.2 Thermo Nicolet, Thermo Fischer Scientific, Waltham, MA, USA) was used for the co-adding and processing of the 64 scans acquired.
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10

Felodipine Miscibility in Polymeric Films

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In order to understand the miscibility and diffusivity of felodipine in the polymeric spin coated films and the possibility of drug-polymer interaction, drug particles on uncoated glass coverslips as a control, or spin coated polymer films (Eudragit E PO, PVPVA, Soluplus or HEC) representing miscible, partially miscible or immiscible binary systems respectively were heated at 20 ºC/min from 30-140 ºC and then kept isothermal slightly above the melting point of the drug for 15 minutes to allow for the maximum possible interaction between felodipine and the polymeric films. The samples were then analysed using a Nicolet iN10MX infrared microscope (Thermo Fisher Scientific, Madison, WI, US) with 25 μm spatial resolution in transmission mode using the coverslip glass as a reference. With standard 0.17mm glass coverslips the useable spectral range was from 4000 to 1200 cm -1 . IR maps were acquired with 64 scans at 8 cm -1 spectral resolution, and analysed using the Omnic Picta software (Thermo Fisher)
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