Sk mel 2

The SK-MEL-2 and G-361 human malignant melanoma cell lines were obtained from the Korean Cell Line Bank (Seoul, Korea). SK-MEL-2 and G-361 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Welgene, Gyeongsan, Gyeongbuk, Korea) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA) and 100 U/mL penicillin–streptomycin (PS; Gibco, Gaithersburg, MD, USA) at 37 ℃ under 5% CO₂ in a humidified incubator chamber until the experiments were performed. HaCaT cells, a non-tumoral human epidermal cell line, were kindly provided by Prof. Jong Kun Park (Wonkwang University, Iksan, Jeonbuk, Korea). HaCaT cells were maintained in RPMI-1640 medium (Welgene, Gyeongsan, Gyeongbuk, Korea) supplemented with 10% FBS and 100 U/mL of PS at 37 ℃ and 5% CO₂ in a humidified incubator chamber until the next use.

B16F10 (mouse melanoma cell line), SK-MEL-2 (human melanoma cell line), SK-MEL-28 (human melanoma cell line), UACC-257 (human melanoma cell line), and HEK293 (human embryonic kidney cell line) cells were cultured in RPMI (Welgene, Gyeongsan, South Korea) with 10% fetal bovine serum (FBS, RMBIO, Missoula, MT, USA) and 1% antibiotic-antimycotic (Gibco, Grand Island, NY, USA). All cells were maintained at 37 °C with 5% CO₂ in a humidified chamber. UACC-257 was provided by the Chungnam National University Hospital (Daejeon, South Korea). B16F10, HEK293, SK-MEL-2, and SK-MEL-28 cells were purchased from the Korean Cell Line Bank (KCLB, Seoul, South Korea).