The microwell specimens were cut to the proper size, and the samples were washed several times with deionized water and ethanol. After the samples were air-dried, double-sided copper tape was used to attach them to a copper pad, where they were treated with vacuum sputtered platinum (JFC-1600; JEOL Ltd., Tokyo, Japan). The surface structures of both the microwells and MCAs were investigated by scanning electron microscopy (SEM; JSM-7001F; JEOL Ltd., Tokyo, Japan). Before the MCAs were viewed by SEM, the medium was aspirated, and the sample was rinsed with PBS. The MCAs were fixed in 0.1 M sodium cacodylate buffer (SCB; Sigma–Aldrich, St Louis, MO, USA), 1.6% PFA and 2.5% glutaraldehyde (Sigma–Aldrich, St Louis, MO, USA) for 4 h at room temperature and then rinsed with 0.1 M SCB on a shaker table for 10 min. The cells were post-fixed in 1.0% aqueous osmium tetroxide (Sigma–Aldrich, St Louis, MO, USA) in 0.1 M SCB in a dark fume hood for 90 min and then rinsed with 0.1 M SCB and placed on a shaker table for another 10 min. Finally, the samples were serially dehydrated with different concentrations of ethanol (37%, 67%, 95% and 100%) on a shaker table for 10 min each and subjected to critical point drying (CPD 030; Balzers, Liechtenstein, Germany) and vacuum sputtered platinum (JFC-1600; JEOL Ltd., Tokyo, Japan).
Jfc 1600
Manufactured by JEOL
Sourced in Japan, United States, Germany
The JFC-1600 is a high-performance sputtering coater designed for sample preparation in various imaging techniques. It utilizes a magnetron sputtering system to deposit thin, uniform metal or carbon coatings on specimens. The coatings enhance surface conductivity, improving the quality of electron microscopy images.
Automatically generated - may contain errors
Lab products found in correlation
Jsm 6390la, by JEOL (11 mentions)
Jsm 7600f, by JEOL (8 mentions)
Jfd 320, by JEOL (5 mentions)
Jsm 6010la, by JEOL (5 mentions)
Jsm 7500f, by JEOL (5 mentions)
Jsm 6390, by JEOL (4 mentions)
Jsm 6390lv, by JEOL (4 mentions)
Jsm 5610lv, by JEOL (4 mentions)
Jsm 6360la, by JEOL (4 mentions)
Jsm 6510lv, by JEOL (4 mentions)
Jsm 6701f, by JEOL (4 mentions)
Jsm 6380lv, by JEOL (3 mentions)
Fesem 7600f, by JEOL (3 mentions)
Jsm 7800f prime, by JEOL (3 mentions)
Jsm 7001f, by JEOL (2 mentions)
Jsm 6610lv, by JEOL (2 mentions)
Jsm 6390a, by JEOL (2 mentions)
Auto fine coater, by JEOL (2 mentions)
Em cpd300, by Leica (2 mentions)
Jsm 6700f, by JEOL (2 mentions)
172 protocols using jfc 1600
1
Scanning Electron Microscopy Characterization of Microwell Specimens
2
Corneal Epithelial Surface Examination
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At different time points after exposure, the rabbit corneas were obtained after sacrifice. The corneas were carefully excised, fixed in 4% glutaraldehyde in 0.05 M cacodylate buffer for 1 hour, and then postfixed in 1% osmium tetroxide in veronal acetate buffer containing 0.22 M sucrose. The fixed materials were dehydrated through a series of ethanol washes. The corneas were placed in t-butyl alcohol, treated in a freeze-drying apparatus (EIKO ID-2; EIKO, Tokyo, Japan), and then sputter coated with gold using an auto fine coater (JEOL JFC-1600; JEOL, Tokyo, Japan). After processing, the surface of the corneal epithelium was observed by means of a SEM microscope (Hitachi SU8220; Hitachi, Ibaragi, Japan).
3
Microwell Surface Characterization by SEM
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The microwell samples were cut into appropriate sizes and rinsed several times with deionized water and ethanol. The samples were air‐dried and then treated with vacuum‐sputtered platinum (JEOL JFC‐1600; JEOL Ltd., Tokyo, Japan). The surfaces of the microwell structures were then observed by SEM (JSM‐7001F; JEOL Ltd.).
4
Surface Morphology Imaging Protocol
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The JEOL JSM 6390 (JEOL, Tokyo, Japan) was used to conduct surface morphology observations. On aluminum stubs, samples were taped together with double-sided carbon tape. The images were analyzed at a 10 kV accelerating voltage after all specimens were sputtered with a thin layer of gold in an auto fine coater JEOL JFC 1600 (JEOL, Tokyo, Japan).
5
Scanning Electron Microscopy of Rabbit Corneas
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Rabbits were anesthetized with an intramuscular injection of 30 mg/kg ketamine and 5 mg/kg xylazine. They were then sacrificed using a lethal dose of intravenous sodium pentobarbital (Nembutal, Dainippon Pharmaceutical, Osaka, Japan) and prepared for SEM examinations. The corneas were carefully excised, fixed in 4% glutaraldehyde in 0.05 M cacodylate buffer for 1 hour, and then post-fixed in 1% osmium tetroxide in veronal acetate buffer containing 0.22 M sucrose. The fixed materials were dehydrated through a series of ethanol washes. Corneas were placed in t-butyl alcohol, treated in a freeze-drying apparatus (EIKO ID-2, EIKO, Tokyo, Japan), and then sputter-coated with gold using an auto fine coater (JEOL JFC-1600, JEOL, Tokyo, Japan). The surface of the corneal epithelium was observed by a scanning electron microscope following processing (Hitachi S2360, Hitachi, Ibaragi, Japan).
6
Characterization of Composite Membranes
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Both outer surface and transverse section of the membranes were investigated using a JEOL JSM-6060 LV scanning electron microscope (JEOL Ltd., Akishima-shi, Japan), elementary analysis of the modifier incorporated into the polymer was provided by this manner. Preliminarily, the samples were coated with an ultrathin gold layer at 3 Pa by means of an auto fine coater JEOL JFC-1600 (JEOL Ltd.).
A fine-dispersed powder was obtained from the composite by its grinding under cooling with liquid nitrogen. The powder was researched using a JEOL JEM 1230 transmission electron microscope (JEOL Ltd.).
Micro- and mesopores were determined by means of nitrogen desorption using a Quantachrome Autosorb 6B analyzer (Quantachrome instruments, Boynton Beach, FL, USA). Before the measurements, the samples were vacuumized at 343 K. Bulk density ρb was estimated from mass and geometrical sizes of the membrane, particle density was found with a picnometer (Archimedes) method similar to [29 ]. Total porosity (ε) was calculated as .
A fine-dispersed powder was obtained from the composite by its grinding under cooling with liquid nitrogen. The powder was researched using a JEOL JEM 1230 transmission electron microscope (JEOL Ltd.).
Micro- and mesopores were determined by means of nitrogen desorption using a Quantachrome Autosorb 6B analyzer (Quantachrome instruments, Boynton Beach, FL, USA). Before the measurements, the samples were vacuumized at 343 K. Bulk density ρb was estimated from mass and geometrical sizes of the membrane, particle density was found with a picnometer (Archimedes) method similar to [29 ]. Total porosity (ε) was calculated as .
7
Scanning Electron Microscopy of Rabbit Corneas
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The rabbits were anesthetized with an intramuscular injection of 30 mg/kg ketamine and 5 mg/kg xylazine. Sensor was gently touching the corneas for 5 seconds mimicking what happen during measurement of TER by this new device. Normal rabbits' corneas without exposure to the sensor of the new device were used for comparison. After washing the cornea, the rabbits were immediately sacrificed using a lethal dose of intravenous sodium pentobarbital (Nembutal, Dainippon Pharmaceutical, Osaka, Japan). The corneas were carefully excised, fixed in 4% glutaraldehyde in 0.05 M cacodylate buffer for 1 hour and then post-fixed in 1% osmium tetroxide in veronal acetate buffer containing 0.22 M sucrose. The fixed materials were dehydrated through a series of ethanol washes. Corneas were placed in t-butyl alcohol, treated in a freeze-drying apparatus (EIKO ID-2, EIKO, Tokyo, Japan), and then sputter-coated with gold using an auto fine coater (JEOL JFC-1600, JEOL, Tokyo, Japan). After processing, the surface of the corneal epithelium was observed by a scanning electron microscope (Hitachi S2360, Hitachi, Ibaragi, Japan).
8
Leaf Preparation for SEM Analysis
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The sampled leaves were prepared for SEM analysis as described by Zakaria and Razak64 , with minor modifications. Fresh leaves were collected from the in vitro grown plants and fixed immediately in Bouin’s fixative65 . The leaf samples were kept under vacuum for 4 h and then washed with 1% cacodylate buffer. Post-fixation was done using 1% cacodylate buffered Osmium tetroxide for 2 h. After fixation, samples were dehydrated with graded series of ethanol to absolute ethanol (50% ethanol for 5 min, 70% ethanol for 30 min, 90% ethanol for 30 min, absolute alcohol for 30 min, and each step in two changes at room temperature of 26 ± 2 °C and dried). The dehydrated samples were mounted onto aluminium stubs, coated with a thin layer of gold in an auto sputter fine coater (JFC 1600, Jeol, Japan) and visualized using Scanning Electron Microscope (VEGA-TESCAN-LMU).
9
Scanning Electron Microscopy of Materials
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A scanning electron microscope (JSM 6610-LV, JEOL, Japan, working at 5 kV) was used to morphologically analyze the control and MB300 at magnifications ranging from ×100 to ×5,000. The sample was mounted on an aluminum stub using double-backed cellophane tape after being coated with gold–palladium (60:40 w/w) in an auto fine coater, JEOL JFC-1600.
10
Scanning Electron Microscopy of Ookinetes
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The purified ookinetes were fixed in 2.5% glutaraldehyde solution in 0.1 M phosphate buffer overnight, rinsed three times with PBS, and then postfixed with 1% osmium tetroxide for 2 hours. The fixed samples were dehydrated using a graded acetone series, CO2- dried in a critical-point drying device (K850, Emitech, USA), and gold-coated in a sputter coater (JFC-1600, JEOL, USA) as detailed previously (55 (link)). The samples were imaged in a JSM-6390LV scanning electron microscope.
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