T1-weighted MR images were collected from mice using a 7T horizontal bore small animal magnet (SGRAD 205/120/HD/S, Agilent Technologies). The feedback control system maintained the core mouse body temperature at 37°C by blowing hot air into the magnet. The parameters of MR images include orientation = axial, echo time (TE): 10 ms, repetition time (TR): 717 ms, average = 4, slices = 35, thickness = 0.5 mm, and display matrix (RO×PE) = 256×128.
Sgrad 205 120 hd s
Manufactured by Agilent Technologies
Sourced in United Kingdom
The SGRAD 205/120/HD/S is a lab equipment product from Agilent Technologies. It is a specialized device designed for use in laboratory settings. The core function of this product is to perform specific tasks related to scientific analysis and research, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
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Lab products found in correlation
4 protocols using sgrad 205 120 hd s
1
In Vivo Mouse Brain Imaging
2
Quantitative 3D Tumor Volumetry via MRI
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Tumor size was confirmed with MRI images collected with a 7T horizontal bore small animal MRI system (SGRAD 205/120/HD/S, Agilent Technologies) using a volume-based transmit/receive quadrature radio frequency coil with an inner diameter of 3.5 cm. The animals were given an intra-peritoneal (i.p.) injection of macrocyclic gadolinium chelate (Gadoteridol, ProHance) at a dose of 2.5 mmol Gd/kg body weight. Body temperature was maintained at 37 °C by blowing hot air into the magnet through a feedback control system. ∼12–15 min after injection of gadolinium, transverse T1-weighted sections were acquired with a scout sequence in 3 orthogonal axes to identify tumor location. A 256 × 128 matrix was obtained in 5 min by conventional spin-echo multi-slice pulse sequence using repetition time (TR) = 8.5 ms, echo time (TE) = 2.6 ms, average = 2, in-plane field of view (FOV) = 35 × 35 mm2, 25-mm slab thickness of 1 mm thick interleaved slices with no gap in between. Tumor volume was assessed with the freehand region of interest (ROI) function of NIH Image J software. Areas were measured on each MRI slice (1 mm thickness) and added together to reconstruct the 3D tumor volume.
3
High-Resolution 3D Structural Imaging
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A 120 mm diameter imaging gradient set (SGRAD 205/120/HD/S, Agilent Technologies UK Ltd., Berkshire, UK) was used. A 72 mm birdcage radiofrequency (RF) coil was employed for RF transmission and a quadrature mouse brain surface coil (RAPID, Germany) was used for signal detection. Tuning and matching of the coil was performed manually.
A T2-weighted, three dimensional (3D) fast spin echo (FSE) sequence was implemented for structural imaging with the following parameters: Field-of-view (FOV) = 19.2 × 16.8 × 12.0 mm; resolution = 150 × 150 × 150 μm; repetition time (TR) = 2,500 ms, effective echo time (TEeff) = 43 ms, ETL = 4; NSA = 1. Total imaging time was ~1 h and 30 min.
A T2-weighted, three dimensional (3D) fast spin echo (FSE) sequence was implemented for structural imaging with the following parameters: Field-of-view (FOV) = 19.2 × 16.8 × 12.0 mm; resolution = 150 × 150 × 150 μm; repetition time (TR) = 2,500 ms, effective echo time (TEeff) = 43 ms, ETL = 4; NSA = 1. Total imaging time was ~1 h and 30 min.
4
MRI-Based Tumor Volume Measurement
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We collected MR images to verify tumor size. We used a 7 T horizontal bore small animal MRI system (SGRAD 205/120/HD/S, Agilent Technologies) with a volume-based transmit/receive quadrature radio frequency coil with an inner diameter of 3.5 cm44 (link). Body temperature was maintained at 37 °C by blowing hot air into the magnet through a feedback control system. Transverse T1-weighted sections were acquired with a scout sequence in 3 orthogonal axes to identify tumor location. A 256 × 128 matrix was obtained in 5 min by conventional spin-echo multi-slice pulse sequence using repetition time (TR) = 8.5 ms, echo time (TE) = 2.6 ms, average = 2, in-plane FOV = 35 × 35 mm2, 25 mm slab thickness of 1 mm thick interleaved slices with no gap in between. Tumor volume was assessed with the freehand region of interest (ROI) function of NIH ImageJ software. Areas were measured on each MRI slice (1 mm thickness) and added together to reconstruct the 3D tumor volume.
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