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Act 10

Manufactured by Beckman Coulter
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The ACT 10 is a compact and automated cell counter designed for routine cell analysis. It provides accurate and reliable cell counts and viability assessment for a variety of cell types. The ACT 10 utilizes advanced optical and electronic technologies to deliver consistent and reproducible results.

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Lab products found in correlation

Vetscan hm5, by Abaxis (1 mentions) Vetscan vs2, by Abaxis (1 mentions) Piccolo xpress chemistry analyzer, by Abbott (1 mentions) I stat, by Abbott (1 mentions) Qiamp dna blood mini kit, by Qiagen (1 mentions) Rat serum, by Thermo Fisher Scientific (1 mentions) L selectin cd62l, by BD (1 mentions) Icam 1 cd54, by BD (1 mentions) Attune nxt acoustic focusing cytometer, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Coulter AcT 10 Hematology Analyzer (4 citations) Coulter AcT 10 cell counter (2 citations)

6 protocols using act 10

1

Ebola, Sudan, and Marburg Virus NHP Challenge Studies

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For Ebola NHP challenge studies samples of whole blood were collected in liquid ethylenediaminetetraacetic acid (K3EDTA) and serum tubes. CBCs were performed with the VetScan HM5 (Abaxis Veterinary Diagnostics). Blood biochemistry analyses were performed with the VetScan VS2 (Abaxis Veterinary Diagnostics). For Sudan and Marburg challenge studies whole blood specimens were collected in EDTA tubes and analyzed using a Beckman Coulter AcT 10 hematology analyzer for CBCs. Serum was separated from whole blood specimens using serum separator tubes and chemistries were analyzed using a Piccolo Xpress Chemistry Analyzer (Abbott).
Brannan J.M., He S., Howell K.A., Prugar L.I., Zhu W., Vu H., Shulenin S., Kailasan S., Raina H., Wong G., Rahim M.N., Banadyga L., Tierney K., Zhao X., Li Y., Holtsberg F.W., Dye J.M., Qiu X, & Aman M.J. (2019). Post-exposure immunotherapy for two ebolaviruses and Marburg virus in nonhuman primates. Nature Communications, 10, 105.
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2

Cerebral Malaria: Clinical Assessment

Cited 1 time
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A history and clinical assessment were performed including assignment of Blantyre coma score for cerebral malaria. Clinical assessments measured at baseline and daily included vital signs, routine biochemistry (sodium, chloride, potassium, bicarbonate, urea nitrogen, glucose, calcium, total bilirubin, creatinine, and lactate) by bedside biochemical analyzer (i-STAT, Abbott Point of Care, Princeton, NJ, USA); and complete blood count measured with an automated counter (Beckman-Coulter Act 10, Brea, CA, USA). Demographic and clinical data were collected and managed during the study using the Research Electronic Data Capture (REDCap) tools 26 (link) hosted at Duke University.
Bush M.A., Florence S.M., Yeo T.W., Kalingonji A.R., Chen Y., Granger D.L., Rubach M.P., Anstey N.M., Mwaikambo E.D, & Weinberg J.B. (2021). Degradation of endothelial glycocalyx in Tanzanian children with Falciparum malaria. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(9), e21805.
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3

Hematological and Viral RNA Extraction Protocol

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Hematological data was generated on an ACT 10 Beckmann Coulter using whole EDTA blood. Since “in-house” hematological reference values were unavailable, reference values from Johnson-Delaney, 1994 for white blood cells and platelets [33 (link)] and Adams et al. 2008 for lymphocyte numbers [20 (link)] were utilized. Extractions were performed using Qiagen QiAMP DNA Blood Minikit according to manufacturer’s instructions using 50uL of EDTA blood, except for the heat inactivation step (10 min @ 56°C) in lysis buffer which was extended for 1 hour to ensure inactivation of the virus Quantitative PCR was performed as previously described [10 (link), 34 (link)].
Mucker E.M., Chapman J., Huzella L.M., Huggins J.W., Shamblin J., Robinson C.G, & Hensley L.E. (2015). Susceptibility of Marmosets (Callithrix jacchus) to Monkeypox Virus: A Low Dose Prospective Model for Monkeypox and Smallpox Disease. PLoS ONE, 10(7), e0131742.
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4

Hematological and Clinical Chemistry Analysis

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Hematological data were generated on an ACT 10 Beckmann Coulter using whole EDTA blood. Abaxis Piccolos were used to evaluate clinical chemistries using Abaxis Chem12 or Chem13 reagent disks using serum samples.
Mucker E.M., Shamblin J.D., Goff A.J., Bell T.M., Reed C., Twenhafel N.A., Chapman J., Mattix M., Alves D., Garry R.F, & Hensley L.E. (2022). Evaluation of Virulence in Cynomolgus Macaques Using a Virus Preparation Enriched for the Extracellular Form of Monkeypox Virus. Viruses, 14(9), 1993.
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5

Neutrophil Subset Characterization by Flow Cytometry

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The whole blood white blood cell (WBC) count with differential was assessed using an automated cell counter (Act10, Beckman Coulter, Brea, CA). Red blood cells were lysed for 5 minutes with 2 mL of Ammonium-Chloride-Potassium (ACK) lysing buffer, washed, and labeled for flow cytometry analysis. Cells were treated with Fc-receptor blockage prior to labeling with CD16/CD32 (Mouse BD Fc Block™) (clone 2.4G2 (RUO), BD Pharmingen, San Jose, CA, USA) and 5% rat serum (Invitrogen, Carlsbad, CA) for 10 minutes. Subsequently, labelling antibodies were incubated for another 20 minutes. The cells were washed and analyzed using an Attune® NxT™ Acoustic Focusing Cytometer (Thermo Fisher Scientific, Waltham, MA).
The following fluorescent-labeled antibodies were used for surface and intracellular labeling: Ly6G (clone: 1A-8), Ly6C (clone: AL-21), CD54 (ICAM-1) (clone: 3E2), L-Selectin (CD62L) (clone: MEL-14) all from BD Biosciences, San Jose, CA. Neutrophil (Ly-6G+/Ly-6C+) subsets were characterized using CD62L (L-Selectin) and CD54 (ICAM-1). The CD54(ICAM-1)-/CD62L+ neutrophil phenotype is considered to characterize naïve neutrophils, whereas the CD54(ICAM-1)+/CD62L- neutrophils are considered activated [11 (link),12 (link)].
Salyer C.E., Bergmann C.B., Hotchkiss R.S., Crisologo P.A, & Caldwell C.C. (2022). Functional characterization of neutrophils allows source control evaluation in a murine sepsis model. The Journal of surgical research, 274, 94-101.
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6

Hematology and Chemistry Evaluation

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Hematological data was generated on an ACT 10 Beckmann Coulter using whole EDTA blood. Abaxis Piccolos were used to evaluate clinical chemistries using Abaxis Chem12 or Chem13 reagent disks using serum samples.
Mucker E.M., Shamblin J.D., Raymond J.L., Twenhafel N.A., Garry R.F, & Hensley L.E. (2022). Effect of Monkeypox Virus Preparation on the Lethality of the Intravenous Cynomolgus Macaque Model. Viruses, 14(8), 1741.
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