The ultraviolet-visible-near infrared (UV–VIS–NIR) absorption spectra were recorded by a Cary 5000 UV–VIS–NIR spectrometer (Agilent Technologies, Inc., Santa Clara, CA, USA). The surface morphology of the samples was measured by a JEOL JEM-2100 transmission electron microscopy (TEM) system (JEOL Ltd., Tokyo, Japan), operated at an acceleration voltage of 200 kV. The X-ray diffraction (XRD) patterns were obtained by a Siemens D5005 X-ray powder diffractometer (Siemens, Munich, Germany), equipped with a Cu Kα radiation source, operated at 40 kV and 30 mA. In situ Raman spectra were obtained at room temperature by a Jobin Yvon/HORIBA HR evolution Raman spectrometer (HORIBA, Ltd., Koyoto, Japan), equipped with integral BX 41 confocal microscopy. Raman spectra of MBA molecules in the Au NR–MBA@Cu2O system were accumulated for 30 s and 10 s in the Au NR–MBA system at room temperature. At least 5 Raman measurements were taken for each sample to verify spectral reproducibility. The spectrometer was calibrated by the Raman band of silicon at 520.7 cm−1.
Cary 5000 uv vis nir spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The Cary 5000 UV-Vis-NIR spectrometer is a versatile instrument designed for measuring the absorbance, reflectance, and transmittance of samples across a wide range of wavelengths, from the ultraviolet (UV) to the near-infrared (NIR) region of the spectrum. It provides accurate and reliable data for a variety of applications.
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Lab products found in correlation
Escalab 250, by Thermo Fisher Scientific (3 mentions)
Avance 400, by Bruker (2 mentions)
Labram hr evolution system, by Horiba (2 mentions)
Agilent pl gpc50, by Agilent Technologies (1 mentions)
Cary 60 spectrometer, by Agilent Technologies (1 mentions)
Apex duo, by Bruker (1 mentions)
Apex 2 ccd detector, by Bruker (1 mentions)
J 710 spectrometer, by Jasco (1 mentions)
S 4700 electron microscope, by Hitachi (1 mentions)
Bx41 microscope, by Olympus (1 mentions)
Dra 2500, by Agilent Technologies (1 mentions)
Pl gpc 50 plus, by Agilent Technologies (1 mentions)
Nmr instrument, by Bruker (1 mentions)
Auriga fib sem workstation, by Zeiss (1 mentions)
Q500 thermogravimetric analyzer, by TA Instruments (1 mentions)
Merlin compact, by Zeiss (1 mentions)
Jem 2010f tem, by JEOL (1 mentions)
Aviii hd 400, by Bruker (1 mentions)
Modular compact rheometer mcr 502, by Anton Paar (1 mentions)
Optima 2000 dv, by PerkinElmer (1 mentions)
25 protocols using cary 5000 uv vis nir spectrometer
1
Characterization of Au NR–MBA@Cu2O System
2
Quantifying Dye Concentration in CM-Dye Crystals
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The concentration
of included dye in CM-dye crystals was determined using solution UV–vis
spectroscopy on dissolved crystals. Calibration curves with R2 values of ∼0.99 were established by
preparing standard dye solutions in a range of concentrations, typically
0.0001–0.2 mM depending on the dye solubility. CM-dye samples
were hand-ground, weighed, dissolved in DI water, and their UV–vis
absorbance measured on an Agilent 8453 UV–vis spectrometer
with a wavelength range of 200–800 nm. Average dye concentrations
in CM-dye were determined based on the calibration curve.
Solid
state UV–vis data were collected on hand-ground CM-dye powders
using an Agilent Cary 5000 UV–vis–NIR Spectrometer.
of included dye in CM-dye crystals was determined using solution UV–vis
spectroscopy on dissolved crystals. Calibration curves with R2 values of ∼0.99 were established by
preparing standard dye solutions in a range of concentrations, typically
0.0001–0.2 mM depending on the dye solubility. CM-dye samples
were hand-ground, weighed, dissolved in DI water, and their UV–vis
absorbance measured on an Agilent 8453 UV–vis spectrometer
with a wavelength range of 200–800 nm. Average dye concentrations
in CM-dye were determined based on the calibration curve.
Solid
state UV–vis data were collected on hand-ground CM-dye powders
using an Agilent Cary 5000 UV–vis–NIR Spectrometer.
3
Absorption Spectra of HSA and Antiretrovirals
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The absorption spectra were recorded on an Agilent Cary 5000-UV-Vis-NIR spectrometer (Santa Clara, CA, USA) at room temperature (298 K). Three different spectra were measured in a quartz cell with a 1.0 cm optical pathlength in the 200–800 nm range with PBS as a baseline, namely non-bound HSA solution (10 μM in PBS), antiretroviral drugs (8.2, 10, and 26 μM in PBS), and HSA/antiretrovirals (with a fixed HSA concentration of 10 μM and antiretroviral concentrations of 8.2, 10, and 26 μM in PBS).
4
Characterization of Fulvic Acid Structure
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Cary 5000 UV-Vis-NIR spectrometer (Agilent) was used to collect the diffuse reflectance UV-visible (DR-UV-vis) spectra of the solid samples. Cary 60 spectrometer (Varian) was used to collect absorption spectra for liquid samples. The emission and excitation spectra were recorded on a Quanta Master 4 fluorescence spectrometer using quartz cuvettes loaded with 2 mL of sample
The molecular weight distribution of fulvic acid in DMSO was analyzed with Gel permeation chromatography (Agilent PL-GPC50, Agilent) using a PLgel Olexis column (300 mm × 7.5 mm, Agilent). The mobile phase was DMSO at a flow rate of 1 mL/min, 100 μL injection volume, and molecular weights were determined by comparison with Pullulan standards.
The molecular weight distribution of fulvic acid in DMSO was analyzed with Gel permeation chromatography (Agilent PL-GPC50, Agilent) using a PLgel Olexis column (300 mm × 7.5 mm, Agilent). The mobile phase was DMSO at a flow rate of 1 mL/min, 100 μL injection volume, and molecular weights were determined by comparison with Pullulan standards.
5
Spectroscopic Analysis of Coatings
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A Cary 5000 UV/Vis/NIR spectrometer (Agilent, U.S.A) was used to record the in-line (direct) transmittance and reflectance spectra of CC, CC-LNP, and CC-Ag@LNP films in the range of 200−2800 nm using air as background. The calculation of average spectral absorption (α) at range of 200−2500 nm was calculated according to the following equation48 (link),49 (link): where A(λ ) is spectral absorption, A = 100% T R (T and R are transmittance and reflectance, respectively). is the solar spectral irradiance (W m-2 nm-1), obtained from ASTM standard G173-03.
6
Solid State and Solution UV-Vis Spectroscopy
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Solid state UV–vis
spectroscopy was performed on an Agilent Cary 5000 UV–vis NIR
spectrometer on neat sample powders. The data was acquired in reflectance
mode, which was subsequently converted to a Kubelka–Munk function.
Solution UV–vis spectroscopy was performed on a PerkinElmer
Lambda 25 UV/Vis spectrometer in quartz cuvettes with the analyte
dissolved in water.
spectroscopy was performed on an Agilent Cary 5000 UV–vis NIR
spectrometer on neat sample powders. The data was acquired in reflectance
mode, which was subsequently converted to a Kubelka–Munk function.
Solution UV–vis spectroscopy was performed on a PerkinElmer
Lambda 25 UV/Vis spectrometer in quartz cuvettes with the analyte
dissolved in water.
7
Characterization of Cellulose Nanocrystal Films
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All reagents were purchased from standard suppliers and used without further purification. Aqueous suspensions of CNCs (2 wt%; pH 2.1) prepared from bleached kraft pulp were obtained from FPInnovations (Supplementary Figure 1 ). All materials for polymerization were purged with nitrogen before use. POM images were recorded on an Olympus BX41 microscope with linear polarizers. Transmission polarized UV-visible spectra were collected with an Agilent Cary 5000 UV-vis/NIR spectrometer. The sample was placed between linear polarizers, which were oriented parallel or crossed as indicated in the text and figure captions. CD spectroscopy was performed in transmission mode using a JASCO J-710 spectrometer. SEM was performed using a Hitachi S4700 electron microscope on gold sputter-coated samples. 2D-XRD images were recorded with a Bruker APEX DUO with APEX II CCD Detector using Cu Kα1 X-ray beam with a wavelength (λ) of 0.154 nm at 0.6 mA, 45 kV for 480 s at 60 mm from the detector in transmission mode. Tensile testing was performed on an Instron 5566Q at a rate of 50 mm min−1.
8
Optical Transmittance Spectra of E-Beam Irradiated Samples
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The transmittance spectra in a wavelength range from 200 to 800 nm were obtained using a Cary 5000 UV–VIS-NIR spectrometer from Agilent (Santa Clara, CA, USA) with a step width of 0.5 nm. Directly after E-beam irradiation, the samples were placed on the holder for the measurement of their optical transmittance.
9
Optical Characterization of Nanomaterials
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Reflectance measurements were performed with a Cary 5000 UV–vis–NIR spectrometer (Agilent Technologies) equipped with an integrating sphere (internal DRA 2500). Particles were measured as a dispersion in IPA (c = 2–2.5 g/L) in standard cuvettes made of special optical glass (OS, Hellma) in the range of 400 to 850 nm. At 800 nm, the detector of the instrument changed, which caused a small artifact at this wavelength. While this artifact is visible in Figure 7a , it becomes negligible in Figure 7b and 7c because it corresponds to an energy of 1.55 eV, which is not in a relevant range for the band gap analysis of the measured samples, and the intensity decreases to an invisible level. The estimation of the accuracy of the Tauc method was based on a study by Viezbicke et al., who evaluated the accuracy of the Tauc plot for 120 individual analyses of polycrystalline ZnO and found a deviation of about 0.03 eV [52 (link)].
10
Characterization of Phosphate Block Copolymers
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