BAL was performed in neonatal and adult mice as previously described 25 (link). For microbiology studies, BAL was performed aseptically and colony forming units (CFUs) enumerated were enumerated in serially plated dilutions [plated onto Columbia anaerobe sheep blood agar (Becton Dickinson, NJ) and incubated in a candle jar to facilitate the growth of microaerophilic bacteria at 37°C for 24 hours], as previously described 27 (link). Mouse TNFα, KC, MIP-2, LIX, IL-6, IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM were measured in cell-free BAL using a Luminex-based assay (EMD Millipore, Billerica, MA), according to the manufacturer's instructions.
Luminex based assay
Manufactured by Merck Group
The Luminex-based assay is a multiplex technology that enables the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes color-coded microspheres or beads coated with specific capture molecules to bind and identify target analytes. The captured analytes are then detected using fluorescently labeled reporter molecules. This technology provides a flexible and efficient platform for a wide range of applications, including protein and cytokine profiling, biomarker discovery, and immunoassay development.
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Lab products found in correlation
2 protocols using luminex based assay
1
Bronchoalveolar Lavage for Microbial and Cytokine Analysis
2
Bronchoalveolar Lavage for Microbial and Cytokine Analysis
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BAL was performed in neonatal and adult mice as previously described 25 (link). For microbiology studies, BAL was performed aseptically and colony forming units (CFUs) enumerated were enumerated in serially plated dilutions [plated onto Columbia anaerobe sheep blood agar (Becton Dickinson, NJ) and incubated in a candle jar to facilitate the growth of microaerophilic bacteria at 37°C for 24 hours], as previously described 27 (link). Mouse TNFα, KC, MIP-2, LIX, IL-6, IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM were measured in cell-free BAL using a Luminex-based assay (EMD Millipore, Billerica, MA), according to the manufacturer's instructions.
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