Plasma biochemistry analyses of glucose (GLU), total cholesterol (TC), triglycerides (TG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total protein (TP), blood urea nitrogen (BUN), and creatinine (CRE) were performed using an autoanalyzer (JCA-BM2250, JEOL Ltd., Tokyo, Japan) according to the manufacturer’s protocol. The plasma non-esterified fatty acid (NEFA) concentration was measured using a Wako NEFA-C test commercial kit (Wako Pure Chemical Industries, Inc., Tokyo, Japan). Commercial kits were used for measurement of plasma MDA concentration (NWK-MDA01), SOD activity (NWK-SOD01) and GPx activity (NWK-GPX01, Northwest Life Science Specialties, LLC, Vancouver, Canada). Plasma LDH isozyme pattern was confirmed by a QuickGel LD Isoenzyme kit (Helena Laboratories, Texas, USA) with an Edbank III analysis software (Helena Laboratories, Texas, USA).
Edbank 3 analysis software
Manufactured by Helena Laboratories
Sourced in Japan
Edbank III is a software solution designed for data analysis and management in laboratory settings. It provides core functionality for organizing, processing, and interpreting laboratory data, without making claims about its intended use or specific applications.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using edbank 3 analysis software
1
Comprehensive Plasma Biochemistry Analysis
2
Lipoprotein Profiling by Agarose Gel Electrophoresis
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Serum lipoprotein cholesterol profiles were measured by the biphasic agarose gel electrophoresis method using a commercial kit, Quick gel LIPO gels (Helena Laboratories, Saitama, Japan). Agarose gel fraction bands were analyzed using customized Epalyzer 2 Electrophoresis Processing Analyzer (Helena Laboratories, Saitama, Japan). In brief, 30 μl of each sample was loaded onto the gel, and run with a 14-min migration time at 250 V and 20°C. After migration, the gels underwent a 15-min reaction time, followed by a 12-min decolorizing and a 30-s fixing time. Cholesterol lipoprotein fractions were assessed and analyzed using Edbank III analysis software (Helena Laboratories, Saitama, Japan).
The band of modified LDL on electrophoresis migrates toward the positive electrode when the charge of apo-lipoprotein in LDL is modified. We set normal animals (BCS3) as control and compared the band position of obese animals against each control group.
The band of modified LDL on electrophoresis migrates toward the positive electrode when the charge of apo-lipoprotein in LDL is modified. We set normal animals (BCS3) as control and compared the band position of obese animals against each control group.
3
Electrophoresis Analysis of Protein Expression
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Values are expressed as mean ± SD. Statistical significance was determined by Holm–Sidak One-way ANOVA. All tests were performed using Sigmaplot version.11.2 (Systat Software Inc., San Diego, CA, USA). The significance level was set at p < 0.05. The correlations were determined by Pearson’s product-moment correlation coefficients, r, and are displayed as p < 0.05. The Electrophoresis bands were assessed and analyzed using Edbank III analysis software (Helena Laboratories, Saitama, Japan).
4
Lipoprotein Electrophoresis Protocol
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The blood samples were collected into serum-separating tubes. After a brief centrifugation (2000× g, 30 min) to remove the residual debris of blood cells, the supernatant was prepared for LPEP by isoelectric focusing methods using a commercial kit (Quick gel, Helena Laboratories, Saitama, Japan). Briefly, 30 μL of each blood sample was loaded onto the agarose gel for migration at 250V and 20 °C. Lipoproteins in the blood samples were separated into four major bands on agarose gels based on their electric charges: chylomicron, VLDL (pre-β lipoprotein), LDL (β-lipoprotein), and HDL (α-lipoprotein) [14 (link)]. Each band was assessed and analyzed by densitometric scanning by using Edbank III analysis software (Helena Laboratories, Saitama, Japan) to determine the proportion of each lipoprotein fraction. The presence of chylomicrons in the fasting blood determined by LPEP was an important characteristic feature of HLP5, whereas chylomicrons in the fasting blood was absent in HLP4 [1 (link),5 (link),6 (link)].
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