Log In Sign up
The largest database of trusted experimental protocols

Gsh glo assay

Manufactured by Promega
Visit Supplier
Sourced in United States

The GSH-Glo assay is a luminescent-based method for quantifying glutathione (GSH) in biological samples. The assay utilizes a GSH-specific luciferase substrate that emits light in the presence of GSH, allowing for the measurement of GSH levels.

Automatically generated - may contain errors

Lab products found in correlation

Modulus microplate luminometer, by Promega (3 mentions) Mitopy1, by Bio-Techne (2 mentions) Varioskan microplate reader, by Thermo Fisher Scientific (1 mentions) Spectramax m5 microplate reader, by Molecular Devices (1 mentions) Glutamine glutamate glo assay, by Promega (1 mentions) Synergy ht, by Agilent Technologies (1 mentions) Ros glo assay, by Promega (1 mentions) Infinite m200 microplate reader, by Tecan (1 mentions) Fluorescent plate reader, by Molecular Devices (1 mentions) Passive lysis buffer (plb), by Promega (1 mentions) Synergy h1, by Agilent Technologies (1 mentions) Mitopy1, by Thermo Fisher Scientific (1 mentions) Cm h2dcfda, by Thermo Fisher Scientific (1 mentions) H2dcfda, by Thermo Fisher Scientific (1 mentions) Elisa, by Thermo Fisher Scientific (1 mentions) Envision multilabel plate reader, by PerkinElmer (1 mentions) Mini cordless grinder, by Funakoshi (1 mentions) White 96 well plate, by Greiner (1 mentions)

20 protocols using gsh glo assay

1

Quantifying Cellular Glutathione Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
The GSH-Glo™ assay (Promega, Madison, WI, USA) was used to measure GSH levels. Standard GSH solutions were prepared by diluting a 5 mM stock solution serially (1.56–50 μM) and PBS (0.1 M) was the standard blank. Cells (50 μl/well, 2 × 105 cells/ml) and standards were added into an opaque 96-well plate (duplicate wells), followed by GSH-Glo™ reagent (25 μl/well) and allowed to incubate (30 min, RT) in the dark. Subsequently, luciferin detection reagent (50 μl/well) was added and plates incubated (15 min, RT) in the dark. The absorbance was read on a Modulus™ microplate luminometer (Turner Biosystems, Sunnyvale, USA) and GSH concentrations were calculated by extrapolation from a standard curve.
Naidoo D.B., Chuturgoon A.A., Phulukdaree A., Guruprasad K.P., Satyamoorthy K, & Sewram V. (2017). Centella asiatica modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s). BMC Complementary and Alternative Medicine, 17, 377.
+ Open protocol
+ Expand
2

Quantifying Total Glutathione in Jurkat T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Jurkat T cells were plated at 10,000 cells per well in white 96-well half-area plates. After addition of test SLs or a vehicle (1% DMSO) negative control, the cells were incubated for 30 min at 37 °C, and total GSH was measured using a GSHGlo™ assay (Promega Corp., Madison, WI, USA), according to the manufacturer’s instructions [57 (link)]. Luminescence was measured with a Fluroscan Ascent FL microplate reader.
Khlebnikov A.I., Schepetkin I.A., Kishkentaeva A.S., Shaimerdenova Z.R., Atazhanova G.A., Adekenov S.M., Kirpotina L.N, & Quinn M.T. (2019). Inhibition of T Cell Receptor Activation by Semi-Synthetic Sesquiterpene Lactone Derivatives and Molecular Modeling of Their Interaction with Glutathione and Tyrosine Kinase ZAP-70. Molecules, 24(2), 350.
+ Open protocol
+ Expand
3

Determination of Reduced Glutathione Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reduced glutathione concentration was determined using the GSH-Glo assay (V6911, Promega) according to manufacturer instructions.
Bidault G., Virtue S., Petkevicius K., Jolin H.E., Dugourd A., Guénantin A.C., Leggat J., Mahler-Araujo B., Lam B.Y., Ma M.K., Dale M., Carobbio S., Kaser A., Fallon P.G., Saez-Rodriguez J., McKenzie A.N, & Vidal-Puig A. (2021). SREBP1-induced fatty acid synthesis depletes macrophages antioxidant defences to promote their alternative activation. Nature metabolism, 3(9), 1150-1162.
+ Open protocol
+ Expand
4

Cellular Glutathione Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded and grown to 80% confluency on 96-well plates. Luminescence based GSH-Glo™ Assay (Promega) was used to determine the cellular glutathione (GSH) levels of 100,000 cells on 96-well according to the manufacturer's manual. The luminescence was measured using a Varioskan microplate reader (Thermo Scientific).
Ilter D., Drapela S., Schild T., Ward N.P., Adhikari E., Low V., Asara J., Oskarsson T., Lau E.K., DeNicola G.M., McReynolds M.R, & Gomes A.P. (2023). NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis. Redox Biology, 61, 102627.
+ Open protocol
+ Expand
5

Organoid Glutathione Depletion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The equivalent of 3000 organoids were re-suspended in a 100 μl volume of reduced DMEM-F12 growth medium and plated on 40 μl Matrigel®/BME matrix pre-coated 96-well plates. Organoids were left for 24 h at 37 °C to let the organoids adhere to the matrix, and subsequently treated with control or with BSO for 24 h. GSH concentration was measured using the GSH-Glo assay (Promega) according to the manufacturer’s instructions, and luminescence was measured using SpectraMax M5 microplate reader (Molecular Devices).
Laoukili J., Constantinides A., Wassenaar E.C., Elias S.G., Raats D.A., van Schelven S.J., van Wettum J., Volckmann R., Koster J., Huitema A.D., Nienhuijs S.W., de Hingh I.H., Wiezer R.J., van Grevenstein H.M., Rinkes I.H., Boerma D, & Kranenburg O. (2022). Peritoneal metastases from colorectal cancer belong to Consensus Molecular Subtype 4 and are sensitised to oxaliplatin by inhibiting reducing capacity. British Journal of Cancer, 126(12), 1824-1833.
+ Open protocol
+ Expand
6

Intracellular Glutamate and Glutathione Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the luminescent assays, the MEC-1 and HG-3 cells were seeded in triplicate with CB-839 (0.1-100 μM). After 72 hours of incubation, cells were counted and 50,000 cells per each condition were taken for the luminescent assays. To determine the intracellular glutamate concentrations, a Glutamine/Glutamate-Glo Assay (Promega) was used following the manufacturer’s instructions. The reduced glutathione levels were measured using a GSH-Glo Assay (Promega) according to the manufacturer’s instructions. Luminescence was measured using a BioTek Synergy HT microplate reader. Metabolite concentrations were calculated using a standard curve method.
Timofeeva N., Ayres M.L., Baran N., Santiago-O’Farrill J.M., Bildik G., Lu Z., Konopleva M, & Gandhi V. (2023). Preclinical investigations of the efficacy of the glutaminase inhibitor CB-839 alone and in combinations in chronic lymphocytic leukemia. Frontiers in Oncology, 13, 1161254.
+ Open protocol
+ Expand
7

Quantifying Cellular GSH Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
The GSH-Glo™ assay (Promega, Madison, USA) was performed to measure GSH levels. Standard GSH solutions were prepared by diluting a 5 mM stock solution serially (1.56–50 μM) and PBS (0.1 M) was the standard blank. Cells (50 μl/well, 2 × 105 cells/ml) and standards were added into an opaque 96-well plate, followed by GSH-Glo™ reagent (25 μl/well) and allowed to incubate (30 min, RT) in the dark. Luciferin detection reagent (50 μl/well) was subsequently added and plates incubated (15 min, RT) in the dark. The absorbance was read on a Modulus™ microplate luminometer (Turner Biosystems, Sunnyvale, USA) and GSH concentrations were calculated by extrapolation from a standard curve.
Naidoo D.B., Chuturgoon A.A., Phulukdaree A., Guruprasad K.P., Satyamoorthy K, & Sewram V. (2018). Withania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s). BMC Complementary and Alternative Medicine, 18, 126.
+ Open protocol
+ Expand
8

Quantification of Cellular ROS and GSH

Check if the same lab product or an alternative is used in the 5 most similar protocols
ROS levels were quantified in cells, 3 hours after exposure to CSE (200 μg/ml) or H2O2 (50 mM) through the ROS-Glo Assay according to the manufacturer’s instructions (Promega, Madison, WI). GSH levels were quantified in cells, 6 hours after exposure to CSE (200 μg/ml) or buthionine sulphoximine (BSO) (200 μM) through the GSH-Glo Assay according to the manufacturer’s instructions (Promega). Luminescence was acquired using the Infinite® M200 Microplate reader (Tecan, San Jose, CA, USA). Relative fold changes (FC) in ROS levels were calculated using the following equation; FC = luminescence of sample (treated with DMSO, CSE or H2O2)/luminescence of control sample (treated with DMSO only). Three independent biological replicates were performed for the ROS and GSH quantifications using five to ten technical replicates for each experiment (n = 5–10).
Blake D.J., Reese C.M., Garcia M., Dahlmann E.A, & Dean A. (2015). Increased levels of soluble extracellular Klotho decreases sensitivity to cigarette smoke induced cell death in human lung epithelial cells. Toxicology in vitro : an international journal published in association with BIBRA, 29(7), 1647-1652.
+ Open protocol
+ Expand
9

Assessing Reactive Oxygen Species in SAEC

Check if the same lab product or an alternative is used in the 5 most similar protocols
It is well known that many nanomaterials produce reactive oxygen species that can lead to deleterious effects in cells. Using the CellROX® Green ROS Detection Assay (Invitrogen), we assessed the ability of combustion-derived LCPM to generate reactive oxygen species in SAEC. Cells were exposed to LCPM for 24 h. Following aspiration of suspensions and two washes with 1× PBS, CellROX Green diluted in complete media (5 µM) was added to cells for 30 minutes at 37°C. Fluorescent measurements were then performed using a fluorescent plate reader (Molecular Devices). Total glutathione levels were measured using the GSH-Glo assay according to manufacturer’s instructions (Promega).
Watson-Wright C., Singh D, & Demokritou P. (2016). Toxicological Implications of Released Particulate Matter during Thermal Decomposition of Nano-Enabled Thermoplastics. NanoImpact, 5, 29-40.
+ Open protocol
+ Expand
10

Interferon-γ and Radiotherapy Impact on Glutathione

Check if the same lab product or an alternative is used in the 5 most similar protocols
HT1080 cells were treated with either IFNγ (10 ng/mL), or radiotherapy (16 Gy), or both for 24 hours. Glutathione was quantified using the GSH-Glo assay (Promega). Results normalized to cell viability.
Lang X., Green M.D., Wang W., Yu J., Choi J.E., Jiang L., Liao P., Zhou J., Zhang Q., Dow A., Saripalli A.L., Kryczek I., Wei S., Szeliga W., Vatan L., Stone E.M., Georgiou G., Cieslik M., Wahl D.R., Morgan M.A., Chinnaiyan A.M., Lawrence T.S, & Zou W. (2019). Radiotherapy and immunotherapy promote tumoral lipid oxidation and ferroptosis via synergistic repression of SLC7A11. Cancer discovery, 9(12), 1673-1685.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!