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Annexin 5 alexa fluor 488 ready flow conjugate

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Annexin V Alexa Fluor 488 Ready Flow Conjugate is a fluorescently labeled protein used for the detection of apoptosis in flow cytometry applications. It binds to phosphatidylserine, which is exposed on the surface of cells undergoing programmed cell death.

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Lab products found in correlation

Anti lc3, by Thermo Fisher Scientific (2 mentions) Anti galectin 3, by Santa Cruz Biotechnology (2 mentions) Anti lamp1, by Developmental Studies Hybridoma Bank (2 mentions) Anti c albicans, by GeneTex (2 mentions) Anti goat secondary antibodies conjugated with alexa fluor 488 and horseradish peroxidase hrp, by Thermo Fisher Scientific (2 mentions) Llome l7393, by Merck Group (2 mentions) 3 ma m9281 500mg, by Merck Group (2 mentions) Alexa fluor 647, by Thermo Fisher Scientific (2 mentions) Anti p62, by Cell Signaling Technology (2 mentions) Anti alix, by Santa Cruz Biotechnology (2 mentions) Anti beclin1, by Merck Group (2 mentions) Anti tsg101, by Abcam (2 mentions) Annexin binding buffer, by Thermo Fisher Scientific (2 mentions) Accutase, by Thermo Fisher Scientific (2 mentions) Attune nxt flow cytometer, by Thermo Fisher Scientific (2 mentions) P erk, by GeneTex (1 mentions) P smad2, by GeneTex (1 mentions) P smad3, by GeneTex (1 mentions) Cleaved parp, by GeneTex (1 mentions) Cleaved caspase 3, by GeneTex (1 mentions)

Spelling variants of this product

Alexa Fluor 488 (6806 citations) Alexa 488 (1863 citations) Annexin V (493 citations) Alexa Fluor 488 conjugate (271 citations) Annexin V Alexa Fluor 488 (171 citations) Alexa Fluor conjugates (56 citations) Alexa Fluors (21 citations) Annexin V-Alexa 488 (14 citations) Annexin V Conjugate (6 citations)

5 protocols using annexin 5 alexa fluor 488 ready flow conjugate

1

Curcuminoid Effects on Apoptosis Signaling

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Curcumin (CAS Number 458-37-7, Sigma-Aldrich, Purity: ≧99.5%), demethoxyCurcumin (CAS Number: 22608-11-3, Sigma-Aldrich, Purity: ≧98%), bisdemethoxyCurcumin (CAS Number: 33171-05-0, Sigma-Aldrich, Purity: ≧98%), Thiazolyl Blue Tetrazolium Bromide (MTT) were purchased from Sigma–Aldrich (CAS Number: 298-93-1). β-actin (Cat No. GTX109639), p-p38 (Cat No. GTX24822), p-Akt (Cat No. GTX128414), p-Erk (Cat No. GTX24819), p-smad2 (Cat No. GTX133475), p-smad3 (Cat No. GTX129841), cleaved PARP (Cat No. GTX132329), cleaved caspase 3 (Cat No. GTX86952) antibodies were purchased from GeneTex. Annexin V Alexa Fluor 488 Ready Flow Conjugate (Lot number 2081235) and 7-aminoactinomycin D (7-AAD, Catalog number: A1310) both were purchased from Thermo Fisher Scientific.
Huang C., Lu H.F., Chen Y.H., Chen J.C., Chou W.H, & Huang H.C. (2020). Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and –independent apoptosis via Smad or Akt signaling pathways in HOS cells. BMC Complementary Medicine and Therapies, 20, 68.
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2

Immunoblotting Analysis of Candida Infection

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The primary antibodies are as follows: anti-C. albicans (catalogue #GTX40096, Genetex), anti-LAMP1 (catalogue #1D4B, Developmental Studies Hybridoma Bank), anti-Alix (catalogue #sc-53540 Santa Cruz Biotechnology), anti-Tsg101 (catalogue #AB83 Abcam), anti-Galectin3 (catalogue #sc-32790 Santa Cruz Biotechnology), anti-LC3 (catalogue #PA1-16930 Thermo Fisher Scientific), anti-p62 (catalogue #51145 Cell Signalling), anti-Beclin1 (catalogue #A-00023 Sigma), and anti-Cx43 (catalogue #AB0016–500 SICGEN). Anti-goat secondary antibodies conjugated with Alexa Fluor 488 and horseradish peroxidase (HRP) were purchased from Invitrogen and Life Technologies Jackson, respectively. Anti-mouse secondary antibodies conjugated with Alexa Fluor 568, Alexa Fluor 647 and HRP were purchased from Invitrogen and BioRad, respectively. Anti-rabbit secondary antibodies conjugated with Alexa Fluor 488, Alexa Fluor 647, and HRP were purchased from Invitrogen and BioRad, respectively. Anti-rat secondary antibodies conjugated with Alexa Fluor 594 and HRP were purchased from Invitrogen. Phalloidin was obtained from Sigma-Aldrich/Merck. LLOMe (L7393) and 3-MA (M9281-500MG) were purchased from Sigma-Aldrich/Merck. BAPTA-AM was obtained from Calbiochem (196419). Annexin V Alexa Fluor 488 Ready Flow Conjugate was purchased from Thermo Fisher Scientific (R37174).
Cristovao B., Rodrigues L., Catarino S., Abreu M., Gonçalves T., Domingues N, & Girao H. (2023). Cx43-mediated hyphal folding counteracts phagosome integrity loss during fungal infection. Microbiology Spectrum, 11(5), e01238-23.
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3

Immunoblotting Analysis of Candida Infection

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The primary antibodies are as follows: anti-C. albicans (catalogue #GTX40096, Genetex), anti-LAMP1 (catalogue #1D4B, Developmental Studies Hybridoma Bank), anti-Alix (catalogue #sc-53540 Santa Cruz Biotechnology), anti-Tsg101 (catalogue #AB83 Abcam), anti-Galectin3 (catalogue #sc-32790 Santa Cruz Biotechnology), anti-LC3 (catalogue #PA1-16930 Thermo Fisher Scientific), anti-p62 (catalogue #51145 Cell Signalling), anti-Beclin1 (catalogue #A-00023 Sigma), and anti-Cx43 (catalogue #AB0016–500 SICGEN). Anti-goat secondary antibodies conjugated with Alexa Fluor 488 and horseradish peroxidase (HRP) were purchased from Invitrogen and Life Technologies Jackson, respectively. Anti-mouse secondary antibodies conjugated with Alexa Fluor 568, Alexa Fluor 647 and HRP were purchased from Invitrogen and BioRad, respectively. Anti-rabbit secondary antibodies conjugated with Alexa Fluor 488, Alexa Fluor 647, and HRP were purchased from Invitrogen and BioRad, respectively. Anti-rat secondary antibodies conjugated with Alexa Fluor 594 and HRP were purchased from Invitrogen. Phalloidin was obtained from Sigma-Aldrich/Merck. LLOMe (L7393) and 3-MA (M9281-500MG) were purchased from Sigma-Aldrich/Merck. BAPTA-AM was obtained from Calbiochem (196419). Annexin V Alexa Fluor 488 Ready Flow Conjugate was purchased from Thermo Fisher Scientific (R37174).
Cristovao B., Rodrigues L., Catarino S., Abreu M., Gonçalves T., Domingues N, & Girao H. (2023). Cx43-mediated hyphal folding counteracts phagosome integrity loss during fungal infection. Microbiology Spectrum, 11(5), e01238-23.
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4

Assessing Apoptosis in PC12 Cells

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PC12 cells were seeded in 6 well plates at a density of 2 × 105 cells/well and incubated overnight in a humidified atmosphere (5% CO2) at 37 °C. Cells were pre-treated with ADA for 30 min and with ARs agonist, antagonist, TRR469, and forskolin for 15 min before glutamate exposure for an additional 24 h. At the end of incubation, cells were detached using Accutase (Life Technologies, Monza, Italy) and subsequently stained with Annexin V Alexa Fluor™ 488 Ready Flow Conjugate to 1 × 105 cells in 100 µL of Annexin Binding Buffer (Life Technologies). Cells were then incubated for 5 min at 25 °C, followed by the addition of 1 µM SYTOX™ AADvanced™ Dead Cell Stain. Data were acquired on an Attune NxT Flow Cytometer (Thermo-Fisher Scientific, Paisley, UK) equipped with a 488 nm laser for excitation. Fluorescence emission was collected using a 530/30 BP filter and a 695/40 BP filter for Annexin V Alexa Fluor™ 488 and SYTOX™ AADvanced™, respectively. PC 12 cells were gated according to physical parameters and cell aggregates were removed from the analysis.
Vincenzi F., Pasquini S., Gessi S., Merighi S., Romagnoli R., Borea P.A, & Varani K. (2020). The Detrimental Action of Adenosine on Glutamate-Induced Cytotoxicity in PC12 Cells Can Be Shifted towards a Neuroprotective Role through A1AR Positive Allosteric Modulation. Cells, 9(5), 1242.
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5

Annexin V and SYTOX Apoptosis Assay

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At the end of each treatment, cells were detached using Accutase (Life Technologies) and subsequently stained with Annexin V Alexa Fluor™ 488 Ready Flow Conjugate to 1 x 105 cells in 100 µl of Annexin Binding Buffer (Life Technologies). Cells were then incubated for 5 minutes at 25°C, followed by the addition of 1 µM SYTOX™ AADvanced™ Dead Cell Stain. Data were acquired on an Attune NxT Flow Cytometer (Thermo-Fisher Scientific, Paisley, UK) equipped with a 488 nm laser for excitation. Fluorescence emission was collected using a 530/30 BP filter and a 695/40 BP filter for Annexin V Alexa Fluor™ 488 and SYTOX™ AADvanced™, respectively (36 (link)). Cells were gated according to physical parameters and cell aggregates were removed from the analysis.
Vincenzi F., Rotondo J.C., Pasquini S., Di Virgilio F., Varani K, & Tognon M. (2021). A3 Adenosine and P2X7 Purinergic Receptors as New Targets for an Innovative Pharmacological Therapy of Malignant Pleural Mesothelioma. Frontiers in Oncology, 11, 679285.
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