Vevo 770 high resolution micro imaging system

Manufactured by Fujifilm

Sourced in Canada

The Vevo 770 High-Resolution Micro-Imaging System is a state-of-the-art laboratory equipment designed for high-resolution in vivo imaging. The system utilizes advanced ultrasound technology to capture detailed images and measurements of small animals and samples with high spatial resolution.

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Lab products found in correlation

Rmv 707b, by Fujifilm (1 mentions) Rmv707b scan head, by Fujifilm (1 mentions)

13 protocols using vevo 770 high resolution micro imaging system

Cardiac Function Evaluation After Surgery

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On the 28^th day after surgery, the cardiac function was evaluated using the Vevo 770 high-resolution microimaging system (VisualSonics, Toronto, Canada) as previously described.[10 (link)] The ultrasonic probe frequency was 30 HZ with an ultrasound measurement under the guidance of a two-dimensional image. All test images were used the same ultrasound parameters, and each image was measured at least five consecutive cycles.

Li Y.L., Hao W.J., Chen B.Y., Chen J, & Li G.Q. (2018). Cardiac Fibroblast-Specific Activating Transcription Factor 3 Promotes Myocardial Repair after Myocardial Infarction. Chinese Medical Journal, 131(19), 2302-2309.

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Antitumor Efficacy of HTiO2 NPs in SCC7 Liver Tumor

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To evaluate the antitumour efficacy of HTiO₂ NPs in the SCC7 liver tumour model, tumour-bearing mice were prepared as described above. After 3 days, HTiO₂ NPs were injected into the tail vein of the mice and the mice were divided into three groups: (a) non-treated (3 days), (b) non-treated (10 days), and (c) HTiO₂ NPs + US (10 days). Tumour-bearing mice were treated with US 3 h after intravenous injection. US treatment for liver tumour tissue was performed over a total period of 330 seconds (power: 30 W, frequency: 1.5 MHz, duty cycle: 10%, pulse repetition frequency: 1 Hz, time: 30 seconds, interval: 2 mm). In vivo ultrasound 3D rendering images were obtained using a Vevo770^® High-Resolution Micro-Imaging System (Visualsonics, Toronto, Canada) equipped with a RMV 706 probe (frequency: 40 MHz, power: 100%, RF cycles: 1, sound speed: 1540 m/second, depth: 1 mm, FOV: 10 × 10 mm, frame rate: 11 Hz, 3D step size: 0.102 mm). Results for tumour volume inhibition were obtained using the OV-100 small-animal imaging system. Tumour volumes were calculated as a × b² × 0.54, where a is the largest and b the smallest diameter. Ex vivo images of major organ images were also obtained using the OV-100.

You D.G., Deepagan V.G., Um W., Jeon S., Son S., Chang H., Yoon H.I., Cho Y.W., Swierczewska M., Lee S., Pomper M.G., Kwon I.C., Kim K, & Park J.H. (2016). ROS-generating TiO2 nanoparticles for non-invasive sonodynamic therapy of cancer. Scientific Reports, 6, 23200.

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Cardiac Geometry and Function Assessment

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In vivo cardiac geometry and function was assessed using a Vevo 770 high‐resolution micro‐imaging system (VisualSonics), as previously described.¹⁴ Two weeks after TAC surgery, two‐dimensional and M‐mode imaging were performed. The left ventricle inner diameter during diastole (LVIDd) and left ventricle anterior wall thickness during diastole (LVAWd) of mouse hearts were measured. In addition, left ventricular ejection fraction (EF) and fractional shortening (FS) were evaluated.

Yang H., Wang X., Zhou C., Xiao X., Tian C., Li H., Yin C, & Wang H. (2020). Tripartite motif 10 regulates cardiac hypertrophy by targeting the PTEN/AKT pathway. Journal of Cellular and Molecular Medicine, 24(11), 6233-6241.

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Echocardiography Assessment of Cardiac Function

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Echocardiography was performed blindly with the Vevo 770 High-Resolution Micro-Imaging System (VisualSonics) with a 15-MHz linear-array ultrasound transducer. The left ventricle was assessed in both parasternal long-axis and short-axis views at a frame rate of 120 Hz. End-systole or end-diastole were defined as the phases in which the left ventricle appeared the smallest and largest, respectively, and used for ejection-fraction measurements. To calculate the shortening fraction, left-ventricular end-systolic and end-diastolic diameters were measured from the left-ventricular M-mode tracing with a sweep speed of 50 mm/s at the papillary muscle. B-mode was used for two-dimensional measurements of end-systolic and end-diastolic dimensions. Imaging and calculations were done by an individual who was blinded to the treatment applied to each animal and code was broken only after all data acquired.

Padmanabhan A., Alexanian M., Linares-Saldana R., González-Terán B., Andreoletti G., Huang Y., Connolly A.J., Kim W., Hsu A., Duan Q., Winchester S.A., Felix F., Perez-Bermejo J.A., Wang Q., Li L., Shah P.P., Haldar S.M., Jain R, & Srivastava D. (2020). BRD4 Interacts with GATA4 to Govern Mitochondrial Homeostasis in Adult Cardiomyocytes. Circulation, 142(24), 2338-2355.

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Histological and Echocardiographic Analysis of Cardiac Tissue

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Histological studies were performed as described previously [20 (link)]. Briefly, cardiac tissues were paraffin embedded and sectioned at a thickness of 5 μm for hematoxylin and eosin staining. Hematoxylin and eosin staining were performed according to the manufacturer’s protocols. Echocardiography to assess cardiac function was performed in the mice using a Vevo 770 High-Resolution Micro-Imaging System (Visual Sonics Inc, Toronto, Canada). Mice were anesthetized with 1% isofluorane/ oxygen administered through inhalation. M-mode and two-dimensional measurements were performed as described previously [21 (link)].

Guo Y., Zhang C., Shang F.F., Luo M., You Y., Zhai Q., Xia Y, & Suxin L. (2020). Ketogenic Diet Ameliorates Cardiac Dysfunction via Balancing Mitochondrial Dynamics and Inhibiting Apoptosis in Type 2 Diabetic Mice. Aging and Disease, 11(2), 229-240.

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Echocardiographic Assessment of Left Ventricular Dimensions

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Echocardiography was performed with the Vevo 770 High-Resolution Micro-Imaging System (VisualSonics, Toronto, ON, Canada) with a 15-MHz linear array ultrasound transducer. The LV was assessed in both parasternal long-axis and short-axis views at a frame rate of 120 Hz. End-systole or end-diastole was defined as the phase in which the smallest or largest area of LV, respectively, was obtained. Left ventricular end-systolic diameter (LVDS) and left ventricular end-diastolic diameter (LVDD) were measured from the LV M-mode tracing with a sweep speed of 50 mm/sec. at the papillary muscle level.

Guo C., Deng Y., Liu J, & Qian L. (2014). Cardiomyocyte-specific role of miR-24 in promoting cell survival. Journal of Cellular and Molecular Medicine, 19(1), 103-112.

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Cardiovascular Measurements in Mice

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For cardiovascular measurements, a Vevo770 High-Resolution Micro-Imaging System (VisualSonics, Toronto, ON, Canada) with 30-MHz probe (RMV-707B) was used as previously described.^{35 (link)} Male mice were anesthetized with 3–4% isoflurane, and then switched to 1–1.5% isoflurane mixed with 100% oxygen. At the level of the papillary muscles, two-dimensional-guided M-mode echocardiography was obtained from anterior (+septum) and posterior walls. Mouse systolic blood pressure measurement was recorded by Mouse Blood Pressure System (MRBP-MMC, IITC Life Science Inc, CA, USA) through tail-cuff method after the mouse was trained for 2–3 days until it was comfortable and quiet in the nose cone animal holder. Mouse diastolic blood pressure was calculated by the software MRBP Monitor Version 1.59 (MRBP-MMC, IITC Life Science Inc, CA, USA).

Yue X., Yang X., Lin X., Yang T., Yi X., Dai Y., Guo J., Li T., Shi J., Wei L., Fan G.C., Chen C, & Chang J. (2014). Rnd3 haploinsufficient mice are predisposed to hemodynamic stress and develop apoptotic cardiomyopathy with heart failure. Cell Death & Disease, 5(6), e1284-.

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Echocardiography Measurement of Cardiac Function

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The data of echocardiography was obtained with the Vevo 770 High-Resolution Micro-Imaging System (VisualSonics, Canada) using a 30-MHz image transducer. At first, preparing the chest skin of the experimental mice and anesthetized the mice with 1.25% tribromoethyl aicolaol. Proceed the Echo under the mice physiology condition of ∼500 beats per min heart rate to guarantee the accuracy of cardiac function measurement. Then, the mice were fixed on the operating table (37°C) in a supine position. Two-dimensional image was obtained and collected parameters include left ventricular ejection fraction (EF), left ventricular fractional shortening (FS), left ventricular end diastolic anterior wall thickness (LVAWd), and left ventricular end diastolic posterior wall dimension (LVPWd).

Zhou J.C., Jin C.C., Wei X.L., Xu R.B., Wang R.Y., Zhang Z.M., Tang B., Yu J.M., Yu J.J., Shang S., Lv X.X., Hua F., Li P.P., Hu Z.W., Shen Y.M., Wang F.P., Ma X.Y., Cui B., Geng F.N, & Zhang X.W. (2023). Mesaconine alleviates doxorubicin-triggered cardiotoxicity and heart failure by activating PINK1-dependent cardiac mitophagy. Frontiers in Pharmacology, 14, 1118017.

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Echocardiographic Assessment of Cardiac Function

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Echocardiography was performed blindly using the Vevo 770 High-Resolution Micro-Imaging System (Visual Sonics) with a 15 MHz linear-array ultrasound transducer. The left ventricle was assessed in both parasternal long-axis and short-axis views at a frame rate of 120 Hz. End-systole or end-diastole were defined as the phases in which the left ventricle appeared the smallest and largest, respectively, and used for ejection-fraction measurements. To calculate the shortening fraction, left-ventricular end-systolic and end-diastolic diameters were measured from the left-ventricular M-mode tracing with a sweep speed of 50 mm/s at the papillary muscle as previously reported^{47 (link)}. Imaging and calculations were done by an individual who was blinded to the treatment applied to each animal and this code was broken only after all data was acquired. At the end of the experiments, Animals were sacrificed by barbiturate overdose (150 mg/kg pentobarbital sodium, intraperitoneal injection) and cervical dislocation, and their hearts harvested for histological studies.

Xing S., Tian J.Z., Yang S.H., Huang X.T., Ding Y.F., Lu Q.Y., Yang J.S, & Yang W.J. (2021). Setd4 controlled quiescent c-Kit+ cells contribute to cardiac neovascularization of capillaries beyond activation. Scientific Reports, 11, 11603.

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Murine Echocardiography for Cardiac Function

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Mouse transthoracic echocardiograms were performed as previously
described (Mohamed et al., 2016 ).
Briefly, mice were anesthetized with isoflurane, placed on a heating pad,
and ventral fur shaved with clippers. Echocardiography was performed with
the Vevo 770 High-Resolution Micro-Imaging System (VisualSonics) with a
15-MHz linear-array ultrasound transducer. The left ventricle was assessed
in both parasternal long-axis and short-axis views at a frame rate of
120?Hz. End-systole or end-diastole were defined as the phases in which the
left ventricle appeared the smallest and largest, respectively, and used for
ejection-fraction measurements. To calculate the shortening fraction, left
ventricular end-systolic and end-diastolic diameters were measured from the
left ventricular M-mode tracing with a sweep speed of 50 mm/s at the
papillary muscle. Bmode was used for two-dimensional measurements of
end-systolic and end-diastolic dimensions. The echocardiographer was blinded
to the study groups.

Newman J.C., Covarrubias A.J., Zhao M., Yu X., Gut P., Ng C.P., Huang Y., Haldar S, & Verdin E. (2017). Ketogenic diet reduces mid-life mortality and improves memory in aging mice. Cell metabolism, 26(3), 547-557.e8.

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