Sodium [1-13C]-L-lactate was purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury MA, USA). 2.1-M [1-13C]-L-lactate was prepared in 4:1 w/w water:glycerol with 15-mM OX063 as previously described(15 (link), 16 (link)). A SPINlab™ polarizer (GE Healthcare, Waukesha WI, USA) that operates at 5T and ~0.8 K was used for polarizing [1-13C]-L-lactate. 90 or 180 μL of the lactate sample was placed in a sample vial and assembled with a research fluid path (GE Healthcare), which included 16 mL of dissolution media containing 0.1 g/L of disodium ethylenediaminetetraacetate (Na2EDTA). The sample was dissolved after approximately 5 hours of polarization, yielding ~6.0 mL of 30- or 60-mM hyperpolarized [1-13C]-L-lactate. Liquid polarization level and longitudinal relaxation time (T1) of hyperpolarized [1-13C]-L-lactate were calculated from independent in vitro measurements as described in the Supporting Information .
Research fluid path
Manufactured by GE Healthcare
Sourced in United States
The Research Fluid Path is a laboratory equipment designed to manage and control the movement of fluids in research and scientific applications. It provides a controlled environment for the handling and processing of various liquids, enabling researchers to conduct experiments and analyses with precision and accuracy.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using research fluid path
1
Hyperpolarized [1-13C]-L-Lactate Production
2
Hyperpolarized [1-13C]KIC Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
[1-13C] α-ketoisocaproic acid (KIC) was prepared from [1-13C] α-ketoisocaproic acid sodium salt (Cambridge Isotope Laboratories, Andover, MA, USA) by dissolving in 1-M HCl (pH < 1) and extracting the aqueous layer with diethyl ether. The combined organic layer was dried over anhydrous sodium sulfate, filtered and evaporated to afford [1-13C]KIC (7 M, colorless oil, purity >95%, 94% yield)25 (link). For hyperpolarization, a 70-μL sample of the [1-13C]KIC mixed with 11-mM trityl radical (OX063, Oxford Instruments Molecular Biotools Ltd, Oxfordshire, UK) was placed in a sample vial, which was then assembled with a research fluid path (GE Healthcare, Waukesha, WI, USA) containing 16 mL of dissolution media (0.1 g/L Na2EDTA) in the dissolution syringe. After placed in a SPINlab® polarizer (GE Healthcare), the sample was polarized at ~0.8 K of temperature in a 5 T magnet by irradiating microwaves at a frequency of 139.93 GHz. The polarized sample (3 - 4 hrs) was dissolved and mixed with neutralization media (0.72 M NaOH, 0.4 M Trizma and 0.1 g/L Na2EDTA), resulting in 6.5–7.0 mL of 80-mM [1-13C]KIC solution (pH 7.4–8.2).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!