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Nestin mouse mab

Manufactured by BD
Sourced in Germany

Nestin mouse mAb is a laboratory research antibody product that binds to the nestin protein, a type VI intermediate filament protein. Nestin is commonly used as a marker for neural stem and progenitor cells. This product is intended for use in research applications involving the detection and study of nestin-expressing cells.

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2 protocols using nestin mouse mab

1

Comprehensive Antibody Panel for Neural Lineage

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Commercially obtained monoclonal (mAb) and polyclonal (pAb) primary antibodies used in the study: CTIP2 rat pAb (1:200; Cat. ab18465; Abcam), Cux1 rabbit pAb (1:50; Cat. sc-13024, Santa Cruz), GM130 rat mAb (1:100; Cat. 610823; BD), BAF170 rabbit pAb (1:100; Cat. HPA021213; Sigma), BAF60a mouse mAb (1:200; Cat. 611728; BD), BAF155 rabbit pAb (1:20; Cat. sc-10756; Santa Cruz), BRM rabbit pAb (1:200; Cat. ab15597; Abcam), BRG1 rabbit pAb (1:120; Cat. sc-10768X; Santa Cruz), BAF155 mouse mAb (1:100; Cat. sc-48350X; Santa Cruz), BAF250b mouse mAb (1:100; Cat. WH0057492M1; Sigma), Tbr1 rabbit pAb (1:300; Cat. ab31940; Abcam), α-Catenin rabbit pAb (1:200; Cat. C2081; Sigma), Pax6 mouse mAb (1:100; Developmental Studies Hybridoma Bank), Nestin mouse mAb (1:50; Cat. 611658, BD), Pax6 rabbit pAb (1:200; Cat. PRB-278P; Covance), GFP chicken pAb (1:500; Cat. ab13970; Abcam). Secondary antibodies used were Alexa 488-, Alexa 568-, Alexa 594- and Alexa 647-conjugated IgG (various species, 1:400; Molecular Probes).
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2

Antibodies for Developmental Neuroscience

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The following are the polyclonal (pAb) and monoclonal (mAb) primary antibodies used in this study: Pax6 rabbit pAb (1:200; Covance, Schwerte, Germany), H3K9Ac rabbit pAb (Abcam, Cambridge, UK), Sox2 goat (1:100; Santa Cruz, Dallas, TX, USA), Sox2 mouse mAb (1:100; R&D Systems, Minneapolis, MN, USA), Pax6 mouse mAb (1:100; Developmental Studies Hybridoma Bank, MA, USA), Tbr2 rabbit pAb (1:200; Abcam), H3ac rabbit pAb (Upstate, MA, USA), TNC rabbit pAb (Abcam), AP2γ mouse mAb (1:100; Abcam), PTPRZ1 rabbit pAb (Sigma, St. Louis, MO, USA), BAF155 mouse mAb (1:100; Santa Cruz), BAF155 rabbit pAb (1:20; Santa Cruz), Tbr1 rabbit pAb (1:300; Chemicon, St. Louis, MO, USA), HuCD mouse mAb (1:20; Invitrogen, Schwerte, Germany), Ctip2 rat pAb (1:200; Abcam), Cux1 rabbit pAb (1:100; Santa Cruz), Satb2 mouse mAb (1:200; Abcam), NeuN mouse mAb (Chemicon), Nestin mouse mAb (BD, Heidelberg, Germany), and Kat2a rabbit pAb (Abcam).
Secondary antibodies used were horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:10,000; Covance), HRP-conjugated goat anti-mouse IgG (1:5000; Covance), HRP-conjugated goat anti-rat IgG (1:10,000; Covance), and Alexa 488-, Alexa 568-, Alexa 594-, and Alexa 647-conjugated IgG (various species, 1:400; Molecular Probes, Schwerte, Germany). Antibodies were purchased from the indicated manufacturers in Germany.
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