FFPE tissues were used for the RNA® scope assay. RNA scope® in situ hybridization was implemented with the RNA® scope 2.5 HD Assay–BROWN assay (Advanced Cell Diagnostics, Newark, CA, USA) according to the manufacturer's protocols. The target probe used was Hs‐CCL1 (cat.468541) and the positive control probe used was Hs‐PPIB (cat.313901).
Rna scope 2.5 hd assay brown assay
Manufactured by Advanced Cell Diagnostics
Sourced in United States
The RNAscope® 2.5 HD Assay–BROWN is a sensitive in situ hybridization technique developed by Advanced Cell Diagnostics. It enables the detection and visualization of target RNA molecules within intact cells and tissues.
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Lab products found in correlation
2 protocols using rna scope 2.5 hd assay brown assay
1
RNA Scope Assay for FFPE Tissues
2
Kidney Tissue RNA-ISH Visualization
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RNA-in situ hybridization (RNA-ISH) was performed using the standardized protocol for RNAscope 2.5 HD Assay-Brown assay from Advanced Cell Diagnostics, Inc. (Newark, CA). Briefly, kidney sections were deparaffinized, and antigen retrieval was performed as described in the kit. Sections were then processed for RNA-ISH per the manufacturer's protocol. Slides were finally counterstained with hematoxylin, and sections were mounted. Slides were imaged using an Olympus DP21 camera mounted on an Olympus BX60 microscope equipped with an UPlanFl 40×/0.75 lens. Images were acquired using CellSens software; exposure for all images was identical and processed identically using Photoshop.
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