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Anti f4 80 clone nd

Manufactured by Cell Signaling Technology
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Anti-F4/80 (clone ND) is a laboratory reagent used for the detection and analysis of F4/80 protein, a marker expressed on the surface of mouse macrophages. This product is intended for research use only.

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Lab products found in correlation

Ihc zinc fixative, by BD (2 mentions)

2 protocols using anti f4 80 clone nd

1

Quantitative Histological Analysis of Mouse Pancreata

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Mouse pancreata were fixed in IHC Zinc fixative (BD Pharminogen) for 48 hours and processed for paraffin embedding. Histological, Trichrome, and immunohistochemical staining was performed at the NYU School of Medicine Experimental Pathology Research Laboratory. The following antibodies were used: anti-F4/80 (clone ND, Cell Signaling) and anti-CD3 (clone SP7, Spring Biosciences). Percent acinar area was calculated by dividing the number of pixels that constituted normal acinar area and dividing by the total number of pixels that constituted tissue. Percent trichrome area was calculated by dividing the number of blue pixels by the total number of tissue pixels. F4/80 was calculated by dividing the number of brown pixels by the total number of tissue pixels. CD3 was calculated by counting the number of CD3+ cells per field of view and averaging the number over 15 fields.
Nacarelli T., Lau L., Fukumoto T., Zundell J., Fatkhutdinov N., Wu S., Aird K.M., Iwasaki O., Kossenkov A.V., Schultz D., Noma K.I., Baur J.A., Schug Z., Tang H.Y., Speicher D.W., David G, & Zhang R. (2019). NAD+ metabolism governs the proinflammatory senescence-associated secretome. Nature cell biology, 21(3), 397-407.
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2

Quantitative Histological Analysis of Mouse Pancreata

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse pancreata were fixed in IHC Zinc fixative (BD Pharminogen) for 48 hours and processed for paraffin embedding. Histological, Trichrome, and immunohistochemical staining was performed at the NYU School of Medicine Experimental Pathology Research Laboratory. The following antibodies were used: anti-F4/80 (clone ND, Cell Signaling) and anti-CD3 (clone SP7, Spring Biosciences). Percent acinar area was calculated by dividing the number of pixels that constituted normal acinar area and dividing by the total number of pixels that constituted tissue. Percent trichrome area was calculated by dividing the number of blue pixels by the total number of tissue pixels. F4/80 was calculated by dividing the number of brown pixels by the total number of tissue pixels. CD3 was calculated by counting the number of CD3+ cells per field of view and averaging the number over 15 fields.
Nacarelli T., Lau L., Fukumoto T., Zundell J., Fatkhutdinov N., Wu S., Aird K.M., Iwasaki O., Kossenkov A.V., Schultz D., Noma K.I., Baur J.A., Schug Z., Tang H.Y., Speicher D.W., David G, & Zhang R. (2019). NAD+ metabolism governs the proinflammatory senescence-associated secretome. Nature cell biology, 21(3), 397-407.
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